Takebe N, Tsunokawa Y, Nozawa S, Terada M, Sugimura T
Biochem Biophys Res Commun. 1987 Mar 30;143(3):837-44. doi: 10.1016/0006-291x(87)90325-1.
The E6 and E7 regions of human papillomavirus (HPV) type 16 were present in the DNA samples from cervical cancer cell lines, SKG-IIIa and SKG-IIIb, and those from cervical cancer tissues of three different patients. T601 cells, an NIH3T3 transformant obtained by transfection of DNA from a surgical specimen of a cervical cancer, also contained the E6 and E7 regions. The E6 region of HPV type 16 was expressed as mRNA in SKG-IIIa, SKG-IIIb and T601 cells. The E6 and E7 regions of HPV type 18 were present in the DNA samples from cervical cancer cell lines, SKG-I and SKG-II, and those from cervical cancer tissues of two different patients. SKG-I and SKG-II cells expressed the E6 region of HPV type 18 as mRNAs. These results strongly suggest that the E6 and E7 regions or the sequence surrounding these regions are important for maintaining malignant phenotype of cervical cancer cells.
在来自子宫颈癌细胞系SKG-IIIa和SKG-IIIb以及三名不同患者的子宫颈癌组织的DNA样本中，存在人乳头瘤病毒16型（HPV-16）的E6和E7区域。T601细胞是通过转染子宫颈癌手术标本的DNA获得的NIH3T3转化细胞，也含有E6和E7区域。HPV-16的E6区域在SKG-IIIa、SKG-IIIb和T601细胞中表达为mRNA。在来自子宫颈癌细胞系SKG-I和SKG-II以及两名不同患者的子宫颈癌组织的DNA样本中，存在HPV-18的E6和E7区域。SKG-I和SKG-II细胞将HPV-18的E6区域表达为mRNA。这些结果有力地表明，E6和E7区域或这些区域周围的序列对于维持子宫颈癌细胞的恶性表型很重要。
