Czeglédy J, Póka R, Veress G, Gergely L
Institute of Microbiology, University Medical School, Debrecen, Hungary.
J Clin Microbiol. 1992 Jan;30(1):233-6. doi: 10.1128/jcm.30.1.233-236.1992.
We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical surgery on 35 women were also analyzed. Lymph nodes originating from 19 patients whose cervical biopsy specimens were negative for HPV 16 seemed to lack HPV 16 sequences. For 16 women with positive PCR results for cervical biopsy specimens, 9 of 10 lymph node metastases were positive in the PCR, while 11 of their 36 histologically negative lymph nodes were also shown to contain HPV 16 DNA.
我们采用聚合酶链反应（PCR）检测宫颈癌活检标本及盆腔淋巴结中是否存在人乳头瘤病毒16型（HPV 16）DNA。在检测的75份宫颈标本中，36份（48%）在PCR检测中HPV 16呈阳性。对35名女性在根治性手术中切除的总共65个盆腔淋巴结也进行了分析。来自19名宫颈活检标本HPV 16呈阴性患者的淋巴结似乎缺乏HPV 16序列。对于16名宫颈活检标本PCR结果呈阳性的女性，10个淋巴结转移灶中有9个在PCR检测中呈阳性，而在其36个组织学检查呈阴性的淋巴结中，有11个也被检测出含有HPV 16 DNA。
