Chen Y T, Ding J H
Biochem Biophys Res Commun. 1987 Mar 30;143(3):863-71. doi: 10.1016/0006-291x(87)90329-9.
Two fat soluble vitamins, Vitamins E and K, when added into culture medium, were found to increase aryl hydrocarbon hydroxylase activity in human cultured cells. The extent of induction in a hepatoma-derived cell line (Hep G2) by these vitamins is of similar magnitude to those cells receiving benz[a]anthracene; whereas in a mammary tumor-derived cell line (MCF-7), benz[a]anthracene is the best inducer for the hydroxylase activity. The increase of the hydroxylase activity is associated with increased levels of a specific mRNA coding for polynuclear aromatic hydrocarbons-induced form of cytochrome P-450 with Vitamins E and K treatment. The size of the induced mRNA is 3.3 kilobase which is the same as that of benz[a]anthracene treatment.
两种脂溶性维生素，维生素E和维生素K，添加到培养基中时，被发现可增加人培养细胞中的芳烃羟化酶活性。这些维生素对肝癌衍生细胞系（Hep G2）的诱导程度与接受苯并[a]蒽的细胞相似；而在乳腺肿瘤衍生细胞系（MCF-7）中，苯并[a]蒽是羟化酶活性的最佳诱导剂。维生素E和维生素K处理后，羟化酶活性的增加与编码多环芳烃诱导形式的细胞色素P-450的特定mRNA水平升高有关。诱导的mRNA大小为3.3千碱基，与苯并[a]蒽处理的相同。
