Matucci R, Bennardini F, Sciammarella M L, Baccaro C, Stendardi I, Franconi F, Giotti A
Biochem Pharmacol. 1987 Apr 1;36(7):1059-62. doi: 10.1016/0006-2952(87)90414-x.
We compared the binding properties of [3H]-nitrendipine in heart membranes from normal guinea-pig heart and from hypoxic or hypoxic and reoxygenated heart. The [3H]-nitrendipine binds a single class of high capacity (Bmax 667.2 +/- 105.2) with high affinity (KD 0.14 +/- 0.02) binding sites. By contrast, in membranes of hypoxic and reoxygenated heart the Bmax decreases significantly while it remains unaffected during hypoxia. Xanthinoxidase activity is increased in hypoxic-reoxygenated hearts.
