Bruzik J P, Auble D T, deHaseth P L
Biochemistry. 1987 Feb 10;26(3):950-6. doi: 10.1021/bi00377a040.
A series of promoters with nine base-pair substitutions in the spacer DNA separating the -10 and -35 regions was used to demonstrate that Escherichia coli RNA polymerase is sensitive to events affecting the spacer DNA--a region not directly contacted by the enzyme. The drugs distamycin A and netropsin specifically enhanced the rate of functional complex formation at a promoter bearing a substitution of nonalternating A-T base pairs. The effect is exerted at an early step in the RNA polymerase-promoter interaction. We hypothesize that a drug-induced structural alteration in the spacer DNA occurs, similar to that normally resulting from RNA polymerase binding. These findings are relevant to an understanding of potential mechanisms of transcription activation.
使用一系列在分隔 -10 和 -35 区域的间隔 DNA 中有九个碱基对替换的启动子，来证明大肠杆菌 RNA 聚合酶对影响间隔 DNA 的事件敏感——该区域并非酶直接接触的区域。药物偏端霉素 A 和纺锤菌素在一个带有非交替 A-T 碱基对替换的启动子上，特异性地提高了功能复合物形成的速率。这种效应在 RNA 聚合酶与启动子相互作用的早期阶段发挥作用。我们推测，间隔 DNA 中会发生药物诱导的结构改变，类似于通常由 RNA 聚合酶结合导致的结构改变。这些发现对于理解转录激活的潜在机制具有重要意义。
