Suda T, Tomori N, Yajima F, Sumitomo T, Nakagami Y, Ushiyama T, Demura H, Shizume K
Brain Res. 1987 Mar 10;405(2):247-52. doi: 10.1016/0006-8993(87)90294-0.
A time course study on the changes of rat hypothalamic corticotropin-releasing factor (CRF) levels and ACTH levels in plasma, pituitary and hypothalamus after an acute treatment with reserpine was examined using a rat CRF RIA. The massive and prolonged depletion of hypothalamic norepinephrine and dopamine levels provoked by a single injection of reserpine (2 and 8 mg/kg, i.p.) caused a transient decrease of hypothalamic CRF levels and ACTH levels in the anterior pituitary glands, and an increase in plasma ACTH levels. There was a strong correlation between the depletion of hypothalamic CRF and norepinephrine levels. These results suggest that: acute depletion of hypothalamic norepinephrine levels cause the initial release of CRF that stimulates pituitary ACTH secretion, and the depletion of CRF and ACTH stores at the early stage; and noradrenergic pathways may be involved in the inhibitory mechanism of CRF release.
采用大鼠促肾上腺皮质激素释放因子（CRF）放射免疫分析法，对利血平急性处理后大鼠下丘脑促肾上腺皮质激素释放因子（CRF）水平以及血浆、垂体和下丘脑促肾上腺皮质激素（ACTH）水平的变化进行了时程研究。单次注射利血平（2和8mg/kg，腹腔注射）引起下丘脑去甲肾上腺素和多巴胺水平大量且持久的耗竭，导致下丘脑CRF水平以及垂体前叶ACTH水平短暂下降，同时血浆ACTH水平升高。下丘脑CRF耗竭与去甲肾上腺素水平之间存在强相关性。这些结果表明：下丘脑去甲肾上腺素水平的急性耗竭导致CRF的初始释放，刺激垂体ACTH分泌，以及早期CRF和ACTH储存的耗竭；并且去甲肾上腺素能通路可能参与CRF释放的抑制机制。
