Li Biyun, Huang Xiaoxi, Liu Zheng, Xu Xuefeng, Xiao Huijuan, Zhang Xin, Dai Huaping, Wang Chen
Department of Pulmonary and Critical Care Medicine, Perking University China-Japan Friendship School of Clinical Medicine Beijing 100029, P. R. China.
Department of Medical Research, Beijing Chao-Yang Hospital, Capital Medical University Beijing 100029, P. R. China.
Am J Transl Res. 2018 Sep 15;10(9):2967-2974. eCollection 2018.
Idiopathic pulmonary fibrosis (IPF) is a lethal idiopathic interstitial pulmonary disease characterized by progressive deterioration in lung function that commonly affects eldly people. The pathogenesis of the disease is incompletely understood and therefore lacking effective therapy. Ouabain a digitalis has been reported to be able to suppress lung fibroblast activation via downregulating TGF-β-smad signal pathway . Here, we investigated the effects of ouabain in pulmonary fibrosis in vivo. Pulmonary fibrosis was induced in C57/BL6 mice by a intratracheal instillation of bleomycin (2.0 mg/kg), ouabain (0.6 mg/kg) was given daily via intraperitonealinjection for one week starting at 7 days after intratracheal instillation of bleomycin. Our study showed ouabain significantly reduce α-SMA, fibronectin and collagen I expression in lung fibrosis animal model. Further, ouabain inhibits cells proliferation and promotes apoptosis of lung fibroblasts . In conclusion, our results indicate ouabain a novel effective drug that inhibits lung fibrosis progression.
特发性肺纤维化（IPF）是一种致命的特发性间质性肺疾病，其特征是肺功能进行性恶化，常见于老年人。该疾病的发病机制尚未完全明确，因此缺乏有效的治疗方法。据报道，洋地黄类药物哇巴因能够通过下调TGF-β-smad信号通路来抑制肺成纤维细胞的激活。在此，我们研究了哇巴因在体内对肺纤维化的影响。通过气管内注入博来霉素（2.0mg/kg）在C57/BL6小鼠中诱导肺纤维化，从气管内注入博来霉素7天后开始，每天通过腹腔注射给予哇巴因（0.6mg/kg），持续一周。我们的研究表明，哇巴因可显著降低肺纤维化动物模型中α-SMA、纤连蛋白和I型胶原蛋白的表达。此外，哇巴因可抑制细胞增殖并促进肺成纤维细胞凋亡。总之，我们的结果表明哇巴因是一种抑制肺纤维化进展的新型有效药物。
