Venkataraman M, Scott D W
J Immunol. 1977 Dec;119(6):1879-81.
The numbers and fate of antigen-binding cells (ABC) in neonatal and adult mice rendered tolerant to fluorescein (FL)-labeled heterologous gamma-globulins were studied. Similar numbers of FL-ABC were observed 1 day after tolerogen in both adult and neonatal mouse spleens: by 7 days after tolerization, no FL-ABC were observed in either case. Reinjection with FL-tolerogen at 7 days led to the detection of normal numbers of ABC in adult mice but significantly reduced numbers in neonates. This suggests that neonatal ABC either have been deleted or have failed to resynthesize surface receptors. Two weeks after tolerance induction, spleen cells from these tolerant mice were cultured with Escherichia coli lipopolysaccharide (LPS), a polyclonal B cell mitogen, or with specific antigen. Tolerant adult spleen cells made an equivalent anti-FL response to that of the uninjected controls when stimulated with LPS, but were unresponsive to specific antigenic triggering. In contrast, spleen cells from neonatally tolerized mice were unresponsive to either specific or nonspecific (LPS) stimulation. Thus, these neonatally tolerized spleen cells lose sensitivity to polyclonal-stimulating agents (along with their receptors), or more simply, are deleted.
研究了对荧光素（FL）标记的异源γ球蛋白产生耐受的新生小鼠和成年小鼠中抗原结合细胞（ABC）的数量和命运。在给耐受原后1天，在成年和新生小鼠脾脏中观察到相似数量的FL-ABC：到耐受诱导后7天，在两种情况下均未观察到FL-ABC。在7天时再次注射FL-耐受原导致成年小鼠中检测到正常数量的ABC，但新生小鼠中的数量显著减少。这表明新生ABC要么已被清除，要么未能重新合成表面受体。在耐受诱导两周后，将这些耐受小鼠的脾细胞与大肠杆菌脂多糖（LPS，一种多克隆B细胞有丝分裂原）或特异性抗原一起培养。当用LPS刺激时，耐受的成年脾细胞产生的抗FL反应与未注射对照的反应相当，但对特异性抗原触发无反应。相反，新生期耐受小鼠的脾细胞对特异性或非特异性（LPS）刺激均无反应。因此，这些新生期耐受的脾细胞对多克隆刺激剂（连同其受体）失去敏感性，或者更简单地说，被清除。
