Division of Stem Cell Therapy, Institute of Medical Science, University of Tokyo, Tokyo, 108-8639, Japan.
Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan.
Sci Rep. 2018 Oct 16;8(1):15289. doi: 10.1038/s41598-018-33690-7.
To study development of the conceptus in xenogeneic environments, we assessed interspecies chimera formation as well as tetraploid complementation between mouse and rat. Overall contribution of donor PSC-derived cells was lower in interspecies chimeras than in intraspecies chimeras, and high donor chimerism was associated with anomalies or embryonic death. Organ to organ variation in donor chimerism was greater in interspecies chimeras than in intraspecies chimeras, suggesting species-specific affinity differences among interacting molecules necessary for organogenesis. In interspecies tetraploid complementation, embryo development was near normal until the stage of placental formation, after which no embryos survived.
为了研究胚胎在异种环境中的发育，我们评估了种间嵌合体的形成以及鼠和大鼠之间的四倍体互补。与同种嵌合体相比，种间嵌合体中供体 PSC 衍生细胞的总体贡献较低，并且高供体嵌合率与异常或胚胎死亡有关。种间嵌合体中供体嵌合率的器官间变化大于同种嵌合体，这表明在器官发生过程中相互作用的分子之间存在物种特异性亲和力差异。在种间四倍体互补中，胚胎发育直到胎盘形成阶段接近正常，此后没有胚胎存活。
