Marone G, Siri L, Genovese A, Condorelli M
Int Arch Allergy Appl Immunol. 1987;82(3-4):532-4. doi: 10.1159/000234270.
We have examined the influence of betamethasone (BT) on cyclic AMP (cAMP) metabolism and lysosomal enzyme release from highly purified (approximately equal to 99%) human polymorphonuclear leukocytes (PMNs). Preincubation (1-24 h) of human PMNs with BT (10(-9)-10(-5) M) had no effect on either cAMP content or on beta-glucuronidase release induced by formyl-containing tripeptide (f-met peptide). Preincubation (16-24 h) of PMNs with BT (10(-8)-10(-7) M) dose-dependently potentiated the cAMP accumulation caused by beta-agonists (isoproterenol), adenosine A2/Ra agonist (NECA), prostaglandin E1 (PGE1) and histamine in PMNs. Similarly, BT potentiated the inhibition of f-met peptide-induced beta-glucuronidase release from human PMNs caused by PGE1 (10(-6) M), histamine (2 X 10(-5) M), NECA (10(-4) M) and isoproterenol (10(-6) M).
我们研究了倍他米松（BT）对高纯度（约99%）人多形核白细胞（PMN）中环磷酸腺苷（cAMP）代谢及溶酶体酶释放的影响。人PMN与BT（10⁻⁹ - 10⁻⁵ M）预孵育（1 - 24小时），对cAMP含量或含甲酰基三肽（f - met肽）诱导的β - 葡萄糖醛酸酶释放均无影响。PMN与BT（10⁻⁸ - 10⁻⁷ M）预孵育（16 - 24小时），剂量依赖性地增强了β - 激动剂（异丙肾上腺素）、腺苷A2/Ra激动剂（NECA）、前列腺素E1（PGE1）和组胺在PMN中引起的cAMP积累。同样，BT增强了PGE1（10⁻⁶ M）、组胺（2×10⁻⁵ M）、NECA（10⁻⁴ M）和异丙肾上腺素（10⁻⁶ M）对f - met肽诱导的人PMN中β - 葡萄糖醛酸酶释放的抑制作用。
