3D 化学位移编码 MRI 用于健康志愿者过食方案期间腹部脂肪组织容量和成分定量。
3D Chemical Shift-Encoded MRI for Volume and Composition Quantification of Abdominal Adipose Tissue During an Overfeeding Protocol in Healthy Volunteers.
机构信息
Univ Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR 5220, U1206, F69621, Lyon, France.
Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Centre Hospitalier Lyon Sud, Pierre-Bénite, Lyon, France.
出版信息
J Magn Reson Imaging. 2019 Jun;49(6):1587-1599. doi: 10.1002/jmri.26532. Epub 2018 Oct 16.
BACKGROUND
Overweight and obesity are major worldwide health concerns characterized by an abnormal accumulation of fat in adipose tissue (AT) and liver.
PURPOSE
To evaluate the volume and the fatty acid (FA) composition of the subcutaneous adipose tissue (SAT) and the visceral adipose tissue (VAT) and the fat content in the liver from 3D chemical-shift-encoded (CSE)-MRI acquisition, before and after a 31-day overfeeding protocol.
STUDY TYPE
Prospective and longitudinal study.
SUBJECTS
Twenty-one nonobese healthy male volunteers.
FIELD STRENGTH/SEQUENCE: A 3D spoiled-gradient multiple echo sequence and STEAM sequence were performed at 3T.
ASSESSMENT
AT volume was automatically segmented on CSE-MRI between L2 to L4 lumbar vertebrae and compared to the dual-energy X-ray absorptiometry (DEXA) measurement. CSE-MRI and MR spectroscopy (MRS) data were analyzed to assess the proton density fat fraction (PDFF) in the liver and the FA composition in SAT and VAT. Gas chromatography-mass spectrometry (GC-MS) analyses were performed on 13 SAT samples as a FA composition countermeasure.
STATISTICAL TESTS
Paired t-test, Pearson's correlation coefficient, and Bland-Altman plots were used to compare measurements.
RESULTS
SAT and VAT volumes significantly increased (P < 0.001). CSE-MRI and DEXA measurements were strongly correlated (r = 0.98, P < 0.001). PDFF significantly increased in the liver (+1.35, P = 0.002 for CSE-MRI, + 1.74, P = 0.002 for MRS). FA composition of SAT and VAT appeared to be consistent between localized-MRS and CSE-MRI (on whole segmented volume) measurements. A significant difference between SAT and VAT FA composition was found (P < 0.001 for CSE-MRI, P = 0.001 for MRS). MRS and CSE-MRI measurements of the FA composition were correlated with the GC-MS results (for ndb: r = 0.83 P < 0.001, r = 0.84, P = 0.001; for nmidb: r = 0.74, P = 0.006, r = 0.66, P = 0.020) DATA CONCLUSION: The follow-up of liver PDFF, volume, and FA composition of AT during an overfeeding diet was demonstrated through different methods. The CSE-MRI sequence associated with a dedicated postprocessing was found reliable for such quantification.
LEVEL OF EVIDENCE
1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;49:1587-1599.
背景
超重和肥胖是全球范围内的主要健康问题，其特征是脂肪在脂肪组织（AT）和肝脏中异常积聚。
目的
在 31 天过度喂养方案前后，使用三维化学位移编码（CSE）MRI 采集评估皮下脂肪组织（SAT）和内脏脂肪组织（VAT）的体积和脂肪酸（FA）组成以及肝脏的脂肪含量。
研究类型
前瞻性和纵向研究。
受试者
21 名非肥胖健康男性志愿者。
磁场强度/序列：在 3T 下进行三维扰相梯度多次回波序列和 STEAM 序列。
评估
在 L2 至 L4 腰椎之间的 CSE-MRI 上自动分割 AT 体积，并与双能 X 射线吸收法（DEXA）测量进行比较。分析 CSE-MRI 和磁共振波谱（MRS）数据，以评估肝脏中的质子密度脂肪分数（PDFF）以及 SAT 和 VAT 中的 FA 组成。对 13 个 SAT 样本进行气相色谱-质谱分析（GC-MS），以进行 FA 组成的对比测量。
统计检验
使用配对 t 检验、皮尔逊相关系数和 Bland-Altman 图比较测量值。
结果
SAT 和 VAT 体积显著增加（P<0.001）。CSE-MRI 和 DEXA 测量结果具有很强的相关性（r=0.98，P<0.001）。肝脏的 PDFF 显著增加（CSE-MRI 为+1.35，P=0.002，MRS 为+1.74，P=0.002）。SAT 和 VAT 的 FA 组成在局部 MRS 和 CSE-MRI（整个分割体积）测量中似乎一致。SAT 和 VAT 的 FA 组成存在显著差异（CSE-MRI 为 P<0.001，MRS 为 P=0.001）。MRS 和 CSE-MRI 的 FA 组成测量与 GC-MS 结果相关（对于 ndb：r=0.83，P<0.001，r=0.84，P=0.001；对于 nmidb：r=0.74，P=0.006，r=0.66，P=0.020）。
数据结论
通过不同方法证明了在过度喂养饮食期间对肝脏 PDFF、AT 体积和 FA 组成的随访。与专用后处理相关联的 CSE-MRI 序列被发现对这种定量是可靠的。
证据水平
1 技术功效：第 2 阶段 J. Magn. Reson. Imaging 2019;49:1587-1599.
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