Zavadil Kokáš Filip, Bergougnoux Véronique, Majeská Čudejková Mária
Department of Molecular Biology, Centre of Region Haná for Biotechnological and Agricultural Research, Palacký University in Olomouc, Olomouc, Czech Republic.
J Comput Biol. 2019 Feb;26(2):117-123. doi: 10.1089/cmb.2018.0149. Epub 2018 Oct 16.
Recent technological advances have made next-generation sequencing (NGS) a popular and financially accessible technique allowing a broad range of analyses to be done simultaneously. A huge amount of newly generated NGS data, however, require advanced software support to help both in analyzing the data and biologically interpreting the results. In this article, we describe SATrans (Software for Annotation of Transcriptome), a software package providing fast and robust functional annotation of novel sequences obtained from transcriptome sequencing. Moreover, it performs advanced gene ontology analysis of differentially expressed genes, thereby helping to interpret biologically-and in a user-friendly form-the quantitative changes in gene expression. The software is freely available and provides the possibility to work with thousands of sequences using a standard personal computer or notebook running on the Linux operating system.
最近的技术进步使下一代测序（NGS）成为一种流行且经济上可承受的技术，它能够同时进行广泛的分析。然而，大量新生成的NGS数据需要先进的软件支持，以帮助分析数据并从生物学角度解释结果。在本文中，我们描述了SATrans（转录组注释软件），这是一个软件包，可对从转录组测序获得的新序列进行快速且可靠的功能注释。此外，它还能对差异表达基因进行高级基因本体分析，从而有助于以用户友好的形式从生物学角度解释基因表达的定量变化。该软件可免费获取，使用运行Linux操作系统的标准个人计算机或笔记本电脑就能处理数千条序列。
