Lichtman A H, Williams M E, Ohara J, Paul W E, Faller D V, Abbas A K
J Immunol. 1987 May 15;138(10):3276-83.
A murine helper/inducer T cell clone, D10.G4, has been infected with Kirsten-murine sarcoma virus (KiSV) pseudotyped with an amphotropic murine leukemia virus. The resultant Ki-ras-expressing lines (KiSV-D10) remain dependent on exogenous factors for continued growth but display distinctly different mitotic responses to certain cytokines as compared to the uninfected parent clone. Unlike the parent D10.G4 cells, these KiSV-D10 cells can be maintained in vitro indefinitely in the presence of recombinant interleukin 2 (IL 2), and they all display a maximal proliferative response to purified or recombinant interleukin 1 (IL 1). The IL 1-induced proliferation is shown not to be dependent or secretion of the T cell autocrine growth factors IL 2 or B cell stimulatory factor-1 (BSF-1). The KiSV-D10 lines show certain differences from one another and parent D10.G4 cells in their secretory and proliferative responses to T cell receptor- and BSF-1 mediated signals. These viral oncogene-expressing T cell lines, which remain responsive to and dependent on physiologic growth factors, should prove valuable for analyzing the mechanisms of action of single oncogenes and the intracellular events in T lymphocyte activation.
一株小鼠辅助/诱导性T细胞克隆D10.G4，已被用嗜异性小鼠白血病病毒假型化的 Kirsten 小鼠肉瘤病毒（KiSV）感染。所得表达Ki-ras的细胞系（KiSV-D10）在持续生长方面仍依赖外源性因子，但与未感染的亲代克隆相比，对某些细胞因子表现出明显不同的有丝分裂反应。与亲代D10.G4细胞不同，这些KiSV-D10细胞在重组白细胞介素2（IL-2）存在的情况下可在体外无限期维持，并且它们对纯化的或重组的白细胞介素1（IL-1）均表现出最大增殖反应。结果表明，IL-1诱导的增殖不依赖于T细胞自分泌生长因子IL-2或B细胞刺激因子-1（BSF-1）的分泌。KiSV-D10细胞系在对T细胞受体和BSF-1介导的信号的分泌和增殖反应方面，彼此之间以及与亲代D10.G4细胞存在某些差异。这些表达病毒癌基因的T细胞系，仍对生理生长因子有反应并依赖于它们，对于分析单个癌基因的作用机制以及T淋巴细胞激活中的细胞内事件应该是有价值的。
