Banks L, Spence P, Androphy E, Hubbert N, Matlashewski G, Murray A, Crawford L
J Gen Virol. 1987 May;68 ( Pt 5):1351-9. doi: 10.1099/0022-1317-68-5-1351.
We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.
我们最近报道了18型人乳头瘤病毒（HPV-18）E6蛋白在细菌中的表达以及抗E6多克隆抗体的产生。现在这项工作已扩展到制备一组针对HPV-18 E6蛋白的单克隆抗体。这些抗体表明HPV-16和HPV-18的E6蛋白之间几乎没有抗原保守性，只有一种抗体识别一个交叉反应表位。我们已使用单克隆抗体和多克隆抗体在一些含HPV DNA的细胞系中寻找E6表达。这些试剂特异性地检测到来自人宫颈癌的细胞中有一个16.5K分子量的多肽。
