Vento R, D'Ancona G, Giuliano M, Taibi G, Tesoriere G
J Neurochem. 1987 Jun;48(6):1693-8. doi: 10.1111/j.1471-4159.1987.tb05725.x.
Treatment of chick embryos in ovo with hydrocortisone-21-phosphate (a single dose of 150 micrograms) caused a marked reduction of retinal thymidine kinase activity 24 h later. The inhibitory effect was highest (65-70%) in 8-10-day-old embryos and declined with age, disappearing after day 15. It was accompanied by a reduction in thickness of the retinal layers. Adrenocorticotropic hormone (ACTH) treatment (10 micrograms daily for 2 days) also produced an age-dependent inhibitory effect on retinal thymidine kinase, whereas treatment with a single dose of 200 micrograms of metopirone, a compound that prevents the 11 beta-hydroxylation of steroid molecules in the adrenal glands, impeded the decrease in thymidine kinase activity that normally occurs in chick embryo retina after day 9 of development. In addition, metopirone prevented the inhibition exerted by ACTH on thymidine kinase activity but had no effect on the action of hydrocortisone.
用磷酸氢化可的松21（单剂量150微克）对鸡胚进行卵内处理，24小时后导致视网膜胸苷激酶活性显著降低。在8至10日龄胚胎中，抑制作用最高（65 - 70%），并随年龄增长而下降，在第15天后消失。它伴随着视网膜层厚度的减少。促肾上腺皮质激素（ACTH）处理（每天10微克，共2天）也对视网膜胸苷激酶产生了年龄依赖性抑制作用，而用单剂量200微克美替拉酮处理，美替拉酮是一种能阻止肾上腺中类固醇分子11β - 羟化的化合物，阻碍了鸡胚视网膜在发育第9天后正常发生的胸苷激酶活性下降。此外，美替拉酮阻止了ACTH对胸苷激酶活性的抑制作用，但对氢化可的松的作用没有影响。
