Vento R, D'Ancona G, Taibi G, Giuliano M, Tesoriere G
Institute of Biological Chemistry and Histology, University of Palermo, Italy.
Mech Ageing Dev. 1988 Mar;42(3):239-52. doi: 10.1016/0047-6374(88)90050-4.
The administration in ovo of hydrocortisone-21-phosphate caused, in chick embryo liver, a reduction of the number of hepatocytes which can be isolated from 1 mg dry weight of liver and a marked increase of their size. Moreover, the treatment diminished the incorporation of thymidine into acid-insoluble fraction in these cells whilst it augmented the content of protein, RNA, DNA and the level of thymidine kinase/cell. These effects were highest at 8-10 days, then declined with the age, disappearing after 18th day of incubation. Similar effects were obtained by injecting other glucocorticoids or ACTH. Combined treatment with metopirone abolished the effects found with ACTH, but did not modify the action of hydrocortisone. These findings suggest that glucocorticoids interfere with the proliferative cycle of hepatocytes by inhibiting the mitotic phase and favouring the production of abnormally large cells.
在鸡胚肝脏中卵内注射磷酸氢化可的松-21，导致从1毫克肝脏干重中分离出的肝细胞数量减少，且细胞大小显著增加。此外，该处理减少了这些细胞中胸苷掺入酸不溶性部分的量，同时增加了蛋白质、RNA、DNA的含量以及胸苷激酶/细胞的水平。这些效应在8 - 10天最为明显，随后随年龄增长而下降，在孵化第18天后消失。注射其他糖皮质激素或促肾上腺皮质激素（ACTH）也得到了类似的效应。与甲吡酮联合处理消除了ACTH的效应，但未改变氢化可的松的作用。这些发现表明，糖皮质激素通过抑制有丝分裂期干扰肝细胞的增殖周期，并有利于产生异常大的细胞。
