Tianjin Eye Hospital, Clinic College of Ophthalmology, Tianjin Medical University, Tianjin, China (mainland).
Med Sci Monit. 2018 Oct 17;24:7424-7430. doi: 10.12659/MSM.910601.
BACKGROUND In view of the high incidence of posterior capsule opacification (PCO) and the effects of TGF-β signaling on the epithelial-mesenchymal transition (EMT) of human lens epithelial cells (LECs), our study aimed to explore the mechanism of the function of TGF-β signaling in LECs EMT. MATERIAL AND METHODS Human lens epithelial cells (HLEC-h3) were treated with TGF-β, ILK siRNA, ILK inhibitor, and NF-κB inhibitor to study the effects of TGF-β, ILK, and NF-κB on cell migration and EMT. Cell migration assay was used to measure cell migration ability. Western blot was performed to detect the expression of ILK, E-cadherin, and a-SMA at the protein level. QRT-PCR was used to detect the expression of ILK at the mRNA level. RESULTS Compared with control cells, TGF-β treatment increased the expression level of ILK HLEC-h3, promoted migration of HLEC-h3 cells, increased the expression level of E-cadherin protein, and decreased the expression level of a-SMA protein. However, treatment with ILK siRNA, ILK inhibitor, and NF-κB inhibitor reversed the effects of TGF-β on HLEC-h3 cells. CONCLUSIONS TGF-β-stimulated ILK regulates the migration and EMT of human LECs via NF-κB.
鉴于后囊膜混浊（PCO）的高发病率，以及 TGF-β 信号对人晶状体上皮细胞（LEC）上皮间质转化（EMT）的影响，我们旨在探讨 TGF-β 信号在 LECs EMT 中的功能机制。
用 TGF-β、ILK siRNA、ILK 抑制剂和 NF-κB 抑制剂处理人晶状体上皮细胞（HLEC-h3），以研究 TGF-β、ILK 和 NF-κB 对细胞迁移和 EMT 的影响。细胞迁移实验用于测量细胞迁移能力。Western blot 用于检测 ILK、E-钙黏蛋白和 a-SMA 蛋白的表达水平。QRT-PCR 用于检测 ILK 的 mRNA 表达水平。
与对照组细胞相比，TGF-β 处理增加了 HLEC-h3 中 ILK 的表达水平，促进了 HLEC-h3 细胞的迁移，增加了 E-钙黏蛋白蛋白的表达水平，降低了 a-SMA 蛋白的表达水平。然而，用 ILK siRNA、ILK 抑制剂和 NF-κB 抑制剂处理后，TGF-β 对 HLEC-h3 细胞的作用被逆转。
TGF-β 刺激的 ILK 通过 NF-κB 调节人 LECs 的迁移和 EMT。
