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合浦珠母贝中MicroRNA novel-13和novel-44对副溶血性弧菌感染的应答机制研究

Study on the response mechanism of MicroRNA novel-13 and novel-44 to Vibrio parahaemolyticus infection in Pinctada fucata martensii.

作者信息

Li Chao Jie, Zhang Qi Yuan, Zhang Bin, Liang Hai Ying, Ma Li Ning, Salman Muhammad

机构信息

Fisheries College of Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China.

Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Zhanjiang, Guangdong, 524088, China.

出版信息

BMC Vet Res. 2025 Jan 24;21(1):35. doi: 10.1186/s12917-024-04467-0.

DOI:10.1186/s12917-024-04467-0
PMID:39856657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11760096/
Abstract

Pinctada fucata martensii (P. f. martensii) is one of the main pearl oysters cultured in artificial seawater in China. However, it is highly susceptible to pathogen infection under intensive cultivation near the coast. MicroRNAs (miRNAs), as an innovative and potent regulator of immune function, play a pivotal role in the immune response of pearl oysters to external stimuli and are a potent marker for the response of P. f. martensii to infection. This study identified two novel miRNAs, novel-13 and novel-44, from the whole transcriptome of the P. f. martensii hemocyte before and after infection with Vibrio parahaemolyticus. The dual luciferase results showed that novel-13 negatively regulated LAAO and novel-44 negatively regulated ILK. The activity of antioxidant-related enzymes increased significantly in the synthetic miRNA (novel-13 and novel-44) inhibitors and decreased significantly in the synthetic miRNA mimics. In the challenge experiment, injection with miRNA inhibitor increased the relative survival percentage by 10% compared with the control group. In conclusion, the overexpression of novel-13 and novel-44 can decrease the activity of immune and antioxidant-related enzymes, possibly affecting immune regulation in P. f. martensii by negatively regulating the LAAO and ILK target genes.

摘要

合浦珠母贝是中国人工海水中养殖的主要珍珠贝之一。然而，在近岸集约化养殖条件下，它极易受到病原体感染。微小RNA（miRNA）作为免疫功能的一种创新且强大的调节因子，在珍珠贝对外界刺激的免疫反应中起关键作用，并且是合浦珠母贝对感染反应的有力标志物。本研究从感染副溶血性弧菌前后的合浦珠母贝血细胞全转录组中鉴定出两个新的miRNA，即novel-13和novel-44。双荧光素酶结果表明，novel-13负调控LAAO，novel-44负调控ILK。在合成的miRNA（novel-13和novel-44）抑制剂中，抗氧化相关酶的活性显著增加，而在合成的miRNA模拟物中则显著降低。在攻毒实验中，与对照组相比，注射miRNA抑制剂使相对存活百分比提高了10%。总之，novel-13和novel-44的过表达可降低免疫和抗氧化相关酶的活性，可能通过负调控LAAO和ILK靶基因影响合浦珠母贝的免疫调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/fcf6fb4d17a9/12917_2024_4467_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/21868de2a85a/12917_2024_4467_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/c03d52b2ae92/12917_2024_4467_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/5ef78854be3e/12917_2024_4467_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/fcf6fb4d17a9/12917_2024_4467_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/21868de2a85a/12917_2024_4467_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/fb61c48608f8/12917_2024_4467_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/6ae63d52e901/12917_2024_4467_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/c03d52b2ae92/12917_2024_4467_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/5ef78854be3e/12917_2024_4467_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc24/11760096/fcf6fb4d17a9/12917_2024_4467_Fig6_HTML.jpg

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本文引用的文献

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A Mannose Receptor from Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation.甘露糖受体通过病原体识别和炎症调节参与固有免疫。
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A novel miRNA, Cse-miR-33, functions as an immune regulator by targeting CsTRAF6 in Chinese tongue sole (Cynoglossus semilaevis).
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Comprehensive analysis of the lncRNAs, mRNAs, and miRNAs implicated in the immune response of Pinctada fucata martensii to Vibrio parahaemolyticus.对马氏珠母贝免疫反应中涉及的长链非编码 RNA、信使 RNA 和 microRNA 的综合分析。
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