Suarez C R, Pickett W C, Bell D H, McClintock D K, Oronsky A L, Kerwar S S
J Rheumatol. 1987 Feb;14(1):9-11.
When mediators of inflammation such as complement component C5a or leukotriene B4 are introduced into an air pouch created in mice, these mediators induce the migration of neutrophils into the air pouch. Pretreatment of mice with low doses of methotrexate inhibits leukotriene B4 or C5a induced neutrophil migration into the air pouch. Inhibition of neutrophil chemotaxis by methotrexate may, at least in part, account for the rapid onset of antiinflammatory activity that was observed in clinical trials with methotrexate in rheumatoid arthritis.
当将炎症介质如补体成分C5a或白三烯B4引入小鼠体内创建的气袋中时，这些介质会诱导中性粒细胞迁移至气袋中。用低剂量甲氨蝶呤预处理小鼠可抑制白三烯B4或C5a诱导的中性粒细胞向气袋中的迁移。甲氨蝶呤对中性粒细胞趋化性的抑制作用可能至少部分解释了在类风湿关节炎患者中使用甲氨蝶呤的临床试验中观察到的抗炎活性的快速起效。
