Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry, Beijing, 100097, China.
The Municipal Key Laboratory of Molecular Genetic of Hybrid Wheat, Beijing, 10097, China.
BMC Genomics. 2018 Oct 17;19(1):754. doi: 10.1186/s12864-018-5116-9.
COI (CORONATINE INSENSITIVE), an F-box component of the Skp1-Cullin-F-box protein (SCF) ubiquitin E3 ligase, plays important roles in the regulation of plant growth and development. Recent studies have shown that COIs are involved in pollen fertility. In this study, we identified and characterized COI genes in the wheat genome and analyzed expression patterns under abiotic stress.
A total of 18 COI candidate sequences for 8 members of COI gene family were isolated in wheat (Triticum aestivum L.). Phylogenetic and structural analyses showed that these COI genes could be divided into seven distinct subfamilies. The COI genes showed high expression in stamens and glumes. The qRT-PCR results revealed that wheat COIs were involved in several abiotic stress responses and anther/glume dehiscence in the photoperiod-temperature sensitive genic male sterile (PTGMS) wheat line BS366.
The structural characteristics and expression patterns of the COI gene family in wheat as well as the stress-responsive and differential tissue-specific expression profiles of each TaCOI gene were examined in PTGMS wheat line BS366. In addition, we examined SA- and MeJA-induced gene expression in the wheat anther and glume to investigate the role of COI in the JA signaling pathway, involved in the regulation of abnormal anther dehiscence in the PTGMS wheat line. The results of this study contribute novel and detailed information about the TaCOI gene family in wheat and could be used as a benchmark for future studies of the molecular mechanisms of PTGMS in other crops.
COI（冠菌素不敏感）是 Skp1-Cullin-F-box 蛋白（SCF）泛素 E3 连接酶的 F-box 组件，在植物生长和发育的调控中发挥重要作用。最近的研究表明，COIs 参与花粉育性。在本研究中，我们鉴定并分析了小麦基因组中的 COI 基因，并分析了其在非生物胁迫下的表达模式。
共分离到小麦（Triticum aestivum L.）8 个 COI 基因家族成员的 18 个 COI 候选序列。系统发育和结构分析表明，这些 COI 基因可分为七个不同的亚家族。COI 基因在雄蕊和颖片中表达量较高。qRT-PCR 结果表明，小麦 COIs 参与了几种非生物胁迫反应和光温敏雄性不育小麦系 BS366 的花药/颖壳开裂。
本研究在光温敏雄性不育小麦系 BS366 中对 COI 基因家族在小麦中的结构特征和表达模式，以及每个 TaCOI 基因的胁迫响应和组织特异性差异表达谱进行了研究。此外，我们还研究了 SA 和 MeJA 诱导的小麦花药和颖壳中的基因表达，以研究 COI 在 JA 信号通路中的作用，该通路参与调控光温敏雄性不育小麦系中花药异常开裂。本研究结果为小麦 TaCOI 基因家族提供了新的详细信息，可为其他作物中光温敏雄性不育的分子机制的研究提供参考。
