杆状病毒载体可以表达含内含子的基因。
A baculovirus vector can express intron-containing genes.
作者信息
Jeang K T, Holmgren-Konig M, Khoury G
出版信息
J Virol. 1987 May;61(5):1761-4. doi: 10.1128/JVI.61.5.1761-1764.1987.
A simjan virus 40 genomic fragment containing the genes coding for the large T and small t antigens was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus downstream of the strong polyhedrin promoter. Infection of eucaryotic Spodoptera frugiperda (SF9) cells with this recombinant virus produced significant amounts of small t antigen and little or no large T protein. Analysis by Northern blotting and S1 nuclease digestion revealed correct and preferential utilization of the small t splicing signals.
一个包含编码大T抗原和小t抗原基因的猿猴病毒40基因组片段，被插入到苜蓿银纹夜蛾核型多角体病毒的基因组中，位于强多角体蛋白启动子的下游。用这种重组病毒感染真核草地贪夜蛾（SF9）细胞，产生了大量的小t抗原，而大T蛋白很少或没有。通过Northern印迹法和S1核酸酶消化分析表明，小t剪接信号得到了正确且优先的利用。
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