Solnick D
Cell. 1981 Apr;24(1):135-43. doi: 10.1016/0092-8674(81)90509-2.
An adenovirus-SV40 recombinant has been constructed in which the SV40 early region, joined to a copy of the adenovirus major late promoter, was substituted for a small portion of the adenovirus late region and all of early region 3. The inserted sequence functions as a separate transcriptional unit, using the ectopic adenovirus promoter, SV40 splice sites and SV40 polyadenylation site. The RNAs produced lack both the second and third members of the tripartite leader normally found on adenovirus late RNA, yet are translationally active, directing the synthesis of substantial amounts of SV40 T antigen. The recombinant may serve as a model vehicle for the abundant expression of other eucaryotic genes inserted into the adenovirus genome.
构建了一种腺病毒 - SV40重组体，其中与腺病毒主要晚期启动子拷贝相连的SV40早期区域取代了腺病毒晚期区域的一小部分和整个早期区域3。插入序列作为一个独立的转录单元发挥作用，利用异位腺病毒启动子、SV40剪接位点和SV40聚腺苷酸化位点。所产生的RNA既缺乏通常在腺病毒晚期RNA上发现的三方前导序列的第二个和第三个成员，但仍具有翻译活性，可指导大量SV40 T抗原的合成。该重组体可作为一种模型载体，用于在腺病毒基因组中大量表达其他真核基因。
