参与伪狂犬病病毒核糖核苷酸还原酶活性的病毒多肽的鉴定。
Identification of viral polypeptides involved in pseudorabies virus ribonucleotide reductase activity.
作者信息
Cohen E A, Paradis H, Gaudreau P, Brazeau P, Langelier Y
出版信息
J Virol. 1987 Jun;61(6):2046-9. doi: 10.1128/JVI.61.6.2046-2049.1987.
We studied pseudorabies virus-induced ribonucleotide reductase and found that it exhibited biochemical properties very similar to those of herpes simplex virus reductase. A polyclonal rabbit antiserum (P9) directed against the carboxy terminus of subunit H2 polypeptide (38,000 daltons) of herpes simplex virus reductase neutralized the pseudorabies virus reductase, as well as the herpes simplex virus isozyme. This serum recognized two pseudorabies virus-specified polypeptides of 34,000 and 110,000 daltons, which may represent the two subunits of the enzyme. Furthermore, as already shown for herpes simplex virus reductase (E. A. Cohen, P. Gaudreau, P. Brazeau, and Y. Langelier, Nature [London] 321:441-443, 1986), we show that the nonapeptide itself specifically inhibited pseudorabies reductase activity.
我们研究了伪狂犬病病毒诱导的核糖核苷酸还原酶，发现它表现出与单纯疱疹病毒还原酶非常相似的生化特性。一种针对单纯疱疹病毒还原酶H2亚基多肽（38,000道尔顿）羧基末端的兔多克隆抗血清（P9）中和了伪狂犬病病毒还原酶以及单纯疱疹病毒同工酶。该血清识别出两种分子量分别为34,000和110,000道尔顿的伪狂犬病病毒特异性多肽，它们可能代表该酶的两个亚基。此外，正如之前对单纯疱疹病毒还原酶的研究所示（E. A. 科恩、P. 高德罗、P. 布拉佐和Y. 朗热利尔，《自然》[伦敦]321:441 - 443，1986），我们表明九肽本身可特异性抑制伪狂犬病还原酶活性。
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