Willoughby K, Bennett M, Williams R A, McCracken C, Gaskell R M
Department of Veterinary Pathology, University of Liverpool Veterinary Field Station, Neston, South Wirral, UK.
Virus Genes. 1997;15(3):203-18. doi: 10.1023/a:1007924419113.
The felid herpesvirus 1 (FHV-1) genes encoding the two ribonucleotide reductase (RR) subunits (RR1, large subunit and RR2, small subunit) were cloned and their nucleotide (nt) sequence determined. The RR1 open reading frame (ORF) is 2358 nts long and is predicted to encode a protein of 786 amino acids (aa). In common with herpesviruses in the Varicellovirus genus of the alphaherpesvirus subfamily, FHV-1 RR1 lacks the N-terminal serine threonine protein kinase region present in herpes simplex virus (HSV)-1 and -2. FHV-1 RR1 has a predicted aa identity of 47-64% with other alphaherpesvirus RR1 peptides, falling to 26-29% for gammaherpesviruses. The RR2 ORF is 996 nts long, predicted to encode a protein of 332 aa and has aa identities of 64-70% with alphaherpesviruses and 38-39% with gammaherpesviruses. Molecular phylogenetic analysis groups FHV-1 with equid herpesviruses 1 and 4 (EHV 1 and 4), pseudorabies virus (PRV) and bovid herpesvirus 1 (BHV 1) within the genus Varicellovirus.
克隆了编码两种核糖核苷酸还原酶（RR）亚基（RR1，大亚基和RR2，小亚基）的猫疱疹病毒1型（FHV-1）基因，并确定了它们的核苷酸（nt）序列。RR1开放阅读框（ORF）长2358个核苷酸，预计编码一个786个氨基酸（aa）的蛋白质。与α疱疹病毒亚科水痘病毒属中的疱疹病毒一样，FHV-1 RR1缺乏单纯疱疹病毒（HSV）-1和-2中存在的N端丝氨酸苏氨酸蛋白激酶区域。FHV-1 RR1与其他α疱疹病毒RR1肽的预测氨基酸同一性为47-64%，对于γ疱疹病毒则降至26-29%。RR2 ORF长996个核苷酸，预计编码一个332个氨基酸的蛋白质，与α疱疹病毒的氨基酸同一性为64-70%，与γ疱疹病毒的氨基酸同一性为38-39%。分子系统发育分析将FHV-1与水痘病毒属中的马疱疹病毒1型和4型（EHV 1和4）、伪狂犬病病毒（PRV）和牛疱疹病毒1型（BHV 1）归为一组。
