Wang Bo, Yang Fan, Li Rui, Li Xuemei, Wu Xiaolong, Sun Zheng, Zhai Jieming, He Yan, Qi Jie
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China.
Laizhou Mingbo Aquatic Co., Ltd.
Genes Genet Syst. 2018 Dec 22;93(5):181-190. doi: 10.1266/ggs.18-00009. Epub 2018 Oct 18.
R-spondin2 (Rspo2) is a member of the R-spondin family, which plays important roles in cell proliferation, cell fate determination and organogenesis. Rspo2 exhibits important functions during embryonic development and muscle maintenance in adult human, mouse and Xenopus. In the present study, the tongue sole Cynoglossus semilaevis Rspo2 (CsRspo2) gene was isolated and characterized, and its role in muscle development during embryogenesis was studied. Our results showed that CsRspo2 expression was abundant during gastrulation and significantly high during somite formation, but then decreased markedly after hatching. CsRspo2 expression was high in brain and gill, moderate in heart, ovary and testis, and almost undetectable in muscle and other tissues. Moreover, the potential involvement of Rspo2 in muscle development was investigated. We found that overexpression of CsRspo2 mRNA in zebrafish embryos resulted in slow development and abnormal muscle formation at the embryonic stage. Our work provides a fundamental understanding of the structure and potential functions of CsRspo2 during muscle development.
R-脊椎蛋白2（Rspo2）是R-脊椎蛋白家族的成员，在细胞增殖、细胞命运决定和器官发生中发挥重要作用。Rspo2在人类、小鼠和非洲爪蟾的胚胎发育及成体肌肉维持过程中展现出重要功能。在本研究中，分离并鉴定了半滑舌鳎（Cynoglossus semilaevis）的Rspo2（CsRspo2）基因，并研究了其在胚胎发育过程中对肌肉发育的作用。我们的结果显示，CsRspo2在原肠胚形成期表达丰富，在体节形成期显著高表达，但在孵化后明显下降。CsRspo2在脑和鳃中高表达，在心脏、卵巢和睾丸中中度表达，而在肌肉和其他组织中几乎检测不到。此外，研究了Rspo2在肌肉发育中的潜在作用。我们发现，在斑马鱼胚胎中过表达CsRspo2 mRNA会导致胚胎发育迟缓以及胚胎期肌肉形成异常。我们的工作为CsRspo2在肌肉发育过程中的结构和潜在功能提供了基本认识。
