Tatsumi Yoshiaki, Takeda Moe, Matsuda Masaru, Suzuki Tohru, Yokoi Hayato
Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
Center for Bioscience Research and Education, Utsunomiya University, Utsunomiya 321-8505, Japan.
FEBS Lett. 2014 Dec 20;588(24):4543-50. doi: 10.1016/j.febslet.2014.10.015. Epub 2014 Nov 1.
R-spondin (Rspo) encodes a multi-domain protein that modulates the Wnt-signaling pathway. Two distinct rspo2 zebrafish mutants were generated by TALEN-mediated mutagenesis: a null mutant, rspo2(null), lacking all functional domains, and a hypomorphic mutant, rspo2(tsp), lacking the two N-terminal domains. Mutants were analyzed mainly for abnormalities in the skeletal system. Fin ray skeletons were formed normally in the rspo2(tsp) mutants, but were absent from the rspo2(null) mutants. Hypoplasia of the neural/hemal arches and ribs was observed in both mutants. Thus, the two rspo2 mutants help to identify the functions of Rspo2 in skeletogenesis, as well as functional differences among multiple Rspo2 domains.
R- 信号蛋白（Rspo）编码一种调节 Wnt 信号通路的多结构域蛋白。通过 TALEN 介导的诱变产生了两种不同的 rspo2 斑马鱼突变体：一种是无效突变体 rspo2(null)，缺乏所有功能结构域；另一种是低表达突变体 rspo2(tsp)，缺乏两个 N 端结构域。主要分析了突变体骨骼系统的异常情况。rspo2(tsp) 突变体的鳍条骨骼正常形成，但 rspo2(null) 突变体没有鳍条骨骼。在两种突变体中均观察到神经 / 血弓和肋骨发育不全。因此，这两种 rspo2 突变体有助于确定 Rspo2 在骨骼发生中的功能，以及多个 Rspo2 结构域之间的功能差异。
