Wang Hui, Liu Yan, Liu Wenwen, Cao Mengji, Wang Xifeng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Arch Virol. 2019 Feb;164(2):617-620. doi: 10.1007/s00705-018-4075-4. Epub 2018 Oct 17.
A novel unsegmented chuvirus was found in a leafhopper (Psammotettix alienus) and tentatively named "Tàiyuán leafhopper virus" (TYLeV). The full genome of TYLeV is 14,670 nucleotides in length and includes four ORFs encoding glycoprotein, nucleocapsid protein, RNA-dependent RNA polymerase and an uncharacterized protein, respectively. These genomic features are similar to those of the chuvirus Scaldis River bee virus, but the virus is highly divergent from known chuviruses and other members in the order Mononegavirales, with amino acid sequence identities of only 13.6-33% for the RNA-dependent RNA polymerase (RdRp). On the basis of genomic features, phylogenetic analysis and comparisons of RdRp sequences, we suggest that this virus is a member of a new species of chuvirus in the proposed genus Mivirus.
在一种叶蝉（异域沙叶蝉）中发现了一种新型的不分节段楚病毒，并暂定名为“太原叶蝉病毒”（TYLeV）。TYLeV的全基因组长度为14,670个核苷酸，包含四个分别编码糖蛋白、核衣壳蛋白、RNA依赖性RNA聚合酶和一种未鉴定蛋白的开放阅读框。这些基因组特征与楚病毒斯卡尔迪斯河蜜蜂病毒相似，但该病毒与已知的楚病毒及单股负链RNA病毒目其他成员高度不同，其RNA依赖性RNA聚合酶（RdRp）的氨基酸序列同一性仅为13.6 - 33%。基于基因组特征、系统发育分析以及RdRp序列比较，我们认为该病毒是拟议的米病毒属中一种新的楚病毒物种的成员。
