State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China.
Virus Res. 2020 Aug;285:197992. doi: 10.1016/j.virusres.2020.197992. Epub 2020 May 1.
The leafhopper Psammotettix alienus causes serious economic losses by directly sucking the plant sap and by transmitting plant viruses. Here, we obtained the full-genomic sequence of a novel chuvirus, Hancheng leafhopper mivirus (HCLeV), using RNA sequencing (RNA-seq) and rapid amplification of cDNA ends (RACE). The full genome of HCLeV comprises 9,852 nucleotides (nt) and includes two open reading frames (ORFs), which encode nucleocapsid and large polymerase protein, respectively. Its genomic features are similar to that of Tacheng tick virus 5, a member of chuviruses. However, HCLeV had only 36.6-44.4% identities in amino acid sequence for polymerase and 27.1-44.2% identities for nucleocapsid protein with chuviruses and other viruses in the order Mononegavirales and Jingchuvirales. Interestingly, we found this leafhopper can host two chuvirus species with different genomic structures. The discovery of this new virus potentially adds a new species to the family Chuviridae, and its new genomic structural form indicates broader genomic diversity among the chuviruses.
叶蝉 Psammotettix alienus 通过直接吸食植物汁液和传播植物病毒而造成严重的经济损失。在这里，我们使用 RNA 测序 (RNA-seq) 和快速扩增 cDNA 末端 (RACE) 获得了一种新型呼肠孤病毒，汉成叶蝉 mivirus (HCLeV) 的全基因组序列。HCLeV 的全基因组由 9852 个核苷酸 (nt) 组成，包括两个开放阅读框 (ORF)，分别编码核衣壳蛋白和大聚合酶蛋白。其基因组特征与 Tacheng tick virus 5 相似，后者是呼肠孤病毒的一个成员。然而，HCLeV 在聚合酶和核衣壳蛋白的氨基酸序列上与呼肠孤病毒和单负股病毒目和 Jingchuvirales 目中的其他病毒的同源性分别为 36.6-44.4%和 27.1-44.2%。有趣的是，我们发现这种叶蝉可以携带两种具有不同基因组结构的呼肠孤病毒。这种新病毒的发现可能为 Chuviridae 科增加了一个新物种，其新的基因组结构形式表明呼肠孤病毒之间具有更广泛的基因组多样性。
