Gill A, Timms P, Kemp D J
Mol Biochem Parasitol. 1987 Jan 15;22(2-3):195-202. doi: 10.1016/0166-6851(87)90050-8.
An expression library was constructed by inserting cDNA copied from mRNA of the blood stages of Babesia bovis isolate KA into bacteriophage lambda gt11-amp3. An antigen-positive cDNA clone detected by screening the library with antibodies from cattle vaccinated with the KA isolate was shown to encode part of a high-molecular weight polypeptide antigen of B. bovis. This molecule was a dominant immunogen and was found by immunofluorescence to be within the parasite in infected erythrocytes.
通过将从牛巴贝斯虫KA分离株血液阶段的mRNA拷贝的cDNA插入噬菌体λgt11-amp3构建了一个表达文库。用接种KA分离株的牛的抗体筛选该文库检测到的一个抗原阳性cDNA克隆，被证明编码牛巴贝斯虫的一种高分子量多肽抗原的一部分。该分子是一种主要免疫原，通过免疫荧光发现在感染红细胞的寄生虫内。
