Ciesielski Maciej, Szajewski Mariusz, Pęksa Rafał, Lewandowska Marzena Anna, Zieliński Jacek, Walczak Jakub, Szefel Jarosław, Kruszewski Wiesław Janusz
Department of Oncological Surgery, Gdynia Oncology Centre, Gdynia.
Division of Propedeutics of Oncology, Medical University Of Gdańsk.
Medicine (Baltimore). 2018 Oct;97(42):e12854. doi: 10.1097/MD.0000000000012854.
In gastric cancer, HER2 protein overexpression is considered to be conducive to the higher proliferation activity of the tumor cells. Tumor formation is associated with angiogenesis in order to secure an abundant supply of oxygen and glucose to cancer cells. The aim of the study was to assess if HER2 overexpression is related to higher microvessel density (MVD) in the tumor stroma.The archival samples of primary tumor from 144 consecutive patients that underwent gastric resection for cancer between August 1, 2006 and December 31, 2013 in the Department of Oncological Surgery of Medical University of Gdańsk were analyzed. CD34 was used as a marker of MVD in the tumor stroma. Both CD34 and HER2 protein expressions were tested by immunohistochemistry.The assays were unsuccessful to estimate HER2 in 10 cases and CD34 in 14 cases due to technical reasons. The results were obtained for 128 patients. HER2 0 and HER2 1+ were considered negative, while HER2+ and HER2 3+ were recognized as positive. Mean MVD (mean number of vessels in the visual field) was 32.4 (median 29.5). Microvessel density was insignificantly higher in HER2 positive tumors. The slight difference was also seen between IHC 2+ and 3+ groups. The differences did not reach the level of statistical significance.Statistical analysis performed in our study did not reveal the significant relationship between HER2 overexpression on the tumor cells and MVD in the tumor stroma.
在胃癌中，HER2蛋白过表达被认为有利于肿瘤细胞的更高增殖活性。肿瘤形成与血管生成相关，以便确保向癌细胞充足供应氧气和葡萄糖。本研究的目的是评估HER2过表达是否与肿瘤基质中更高的微血管密度（MVD）相关。对2006年8月1日至2013年12月31日期间在格但斯克医科大学肿瘤外科接受胃癌切除手术的144例连续患者的原发性肿瘤存档样本进行了分析。CD34用作肿瘤基质中MVD的标志物。CD34和HER2蛋白表达均通过免疫组织化学检测。由于技术原因，10例病例的HER2检测和14例病例的CD34检测未成功。获得了128例患者的结果。HER2 0和HER2 1+被视为阴性，而HER2+和HER2 3+被视为阳性。平均MVD（视野中血管的平均数）为32.4（中位数29.5）。HER2阳性肿瘤中的微血管密度略高。在免疫组化2+和3+组之间也观察到细微差异。这些差异未达到统计学意义水平。我们研究中的统计分析未揭示肿瘤细胞上HER2过表达与肿瘤基质中MVD之间的显著关系。
