Arshavskiĭ Iu I, Gel'fand I M, Deliagina T G, Orlovskiĭ G N, Pavlova G A
Neirofiziologiia. 1987;19(1):81-6.
Dissociated neurons from the brain of pteropodial mollusc were cultivated in a 25% Leibovitz medium containing 2% of calf serum. Neurite outgrowth was observed in 1-30% of the neurons. It was maximum during the first 3 days. Neurite length reached 300 microns. Membrane potential of neurons was 40-60 mV; they generated single spikes or bursts of impulses. Intercellular connections were tested on the 3-4th days in 70 pairs of neurons with neurites overlapped. Electrical connections between the cells were observed in 20% of the pairs tested, and in 6% of pairs stimulation of one of the neurons evoked an inhibitory postsynaptic potential in the other.
翼足类软体动物大脑中的解离神经元在含有2%小牛血清的25% Leibovitz培养基中培养。在1% - 30%的神经元中观察到神经突生长。在前3天达到最大值。神经突长度达到300微米。神经元的膜电位为40 - 60 mV；它们产生单个尖峰或冲动爆发。在第3 - 4天，对70对神经突重叠的神经元进行了细胞间连接测试。在20%的测试对中观察到细胞间的电连接，在6%的对中，刺激其中一个神经元会在另一个神经元中诱发抑制性突触后电位。
