Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.
Division of Obstetrics and Gynecology, Department of Obstetrics Rikshospitalet, Oslo University Hospital, Oslo, Norway.
FASEB J. 2019 Feb;33(2):2944-2956. doi: 10.1096/fj.201801193R. Epub 2018 Oct 18.
We sought to identify proteins secreted by the human placenta into the maternal and fetal circulations. Blood samples from the maternal radial artery and uterine vein and umbilical artery and vein were obtained during cesarean section in 35 healthy women with term pregnancy. Slow off-rate modified aptamer (SOMA) protein-binding technology was used to quantify 1310 known proteins. The uteroplacental and umbilical venoarterial concentration differences were calculated. Thirty-four proteins were significantly secreted by the placenta into the maternal circulation, including placental growth factor, growth/differentiation factor 15, and matrix metalloproteinase 12. There were 341 proteins significantly secreted by the placenta into the fetal circulation. Only 7 proteins were secreted into both the fetal and maternal circulations, suggesting a distinct directionality in placental protein release. We examined changes across gestation in the proteins found to be significantly secreted by the placenta into the maternal circulation using serial blood samples from healthy women. Among the 34 proteins secreted into the maternal circulation, 8 changed significantly across gestation. The identified profiles of secreted placental proteins will allow us to identify novel minimally invasive biomarkers for human placental function across gestation and discover previously unknown proteins secreted by the human placenta that regulate maternal physiology and fetal development.-Michelsen, T. M., Henriksen, T., Reinhold, D., Powell, T. L., Jansson, T. The human placental proteome secreted into the maternal and fetal circulations in normal pregnancy based on 4-vessel sampling.
我们试图鉴定人类胎盘分泌到母血和胎血中的蛋白质。在 35 例足月妊娠剖宫产术中，分别从产妇桡动脉和子宫静脉及脐动脉和脐静脉取血。采用慢洗脱率修饰适配体（SOMA）蛋白结合技术定量分析 1310 种已知蛋白质。计算胎盘向母体循环和胎儿循环的分泌浓度差。有 34 种蛋白质显著分泌到胎盘进入母体循环，包括胎盘生长因子、生长/分化因子 15 和基质金属蛋白酶 12。有 341 种蛋白质显著分泌到胎盘进入胎儿循环。仅有 7 种蛋白质分泌到胎儿和母体循环中，表明胎盘蛋白释放具有明显的方向性。我们使用健康女性的系列血样，检查了在妊娠期间被鉴定为显著分泌到母血循环中的蛋白质的变化。在 34 种分泌到母血循环中的蛋白质中，有 8 种在妊娠期间发生显著变化。鉴定出的胎盘分泌蛋白图谱将使我们能够在整个妊娠期间识别新的微创生物标志物，以评估人类胎盘功能，并发现以前未知的调节母体生理和胎儿发育的人类胎盘分泌蛋白。
