At 5D Health Protection Group Ltd, Liverpool, United Kingdom, Anne-Marie Salisbury, PhD, is the Laboratory Manager and Senior Microbiologist; and Steven L. Percival, PhD, is Chief Executive Officer. Acknowledgments: The authors thank the Liverpool Centre for Cell Imaging at the University of Liverpool for their support. 5D Health Protection Group Ltd received a grant and supplies (PluroGel Burn and Wound Dressing and PluroGel with 1% silver sulfadiazine) from Medline Industries Inc to carry out the enclosed research. The authors have disclosed no other financial relationships related to this article. Submitted December 8, 2017; accepted in revised form March 15, 2018.
Adv Skin Wound Care. 2018 Nov;31(11):514-520. doi: 10.1097/01.ASW.0000544612.28804.34.
To assess the biofilm prevention action of two wound dressings, a concentrated surfactant gel preserved with antimicrobials and a concentrated surfactant gel with 1% silver sulfadiazine.
The microorganisms Staphylococcus aureus, methicillin-resistant S aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Enterococcus faecalis were used. Several biofilm models were used whereby the surfaces of each model were coated with either the concentrated surfactant gel preserved with antimicrobials or the concentrated surfactant gel with SSD before biofilm growth.
Results showed the concentrated surfactant gel with SSD prevented biofilm growth in the modified minimum biofilm eradication concentration and Centers for Disease Control and Prevention biofilm models. The concentrated surfactant gel preserved with antimicrobials prevented microbial penetration for up to 48 hours, whereas the concentrated surfactant with SSD prevented microbial penetration for at least 72 hours. Using confocal laser scanning microscopy, researchers showed that a surface coated with the concentrated surfactant gel preserved with antimicrobials enhanced microbial sequestration of planktonic microorganisms.
These results demonstrated that a concentrated surfactant gel preserved with antimicrobials can sequester and cause the immobilization of planktonic bacteria. Further, the concentrated surfactant gel with SSD can effectively kill planktonic and sessile microorganisms.
评估两种伤口敷料的生物膜预防作用，一种是含有抗菌剂的浓缩表面活性剂凝胶，另一种是含有 1%磺胺嘧啶银的浓缩表面活性剂凝胶。
使用金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌和粪肠球菌等微生物。使用了几种生物膜模型，其中每个模型的表面都涂有含有抗菌剂的浓缩表面活性剂凝胶或含有 SSD 的浓缩表面活性剂凝胶，然后再进行生物膜生长。
结果表明，SSD 浓缩表面活性剂凝胶可预防改良最小生物膜清除浓度和疾病预防控制中心生物膜模型中的生物膜生长。含有抗菌剂的浓缩表面活性剂凝胶可防止微生物渗透长达 48 小时，而 SSD 浓缩表面活性剂凝胶可防止微生物渗透至少 72 小时。通过共聚焦激光扫描显微镜，研究人员表明，涂有含有抗菌剂的浓缩表面活性剂凝胶的表面增强了浮游微生物的微生物隔离作用。
这些结果表明，含有抗菌剂的浓缩表面活性剂凝胶可以隔离并固定浮游细菌。此外，含有 SSD 的浓缩表面活性剂凝胶可以有效地杀死浮游和定殖微生物。
