School of Animal Science, Yangtze University, Jingzhou, 434020, China; Guangxi Key Laboratory of Marine Biotechnology, Guangxi Institute of Oceanology, Beihai, 536006, China; State Key Laboratory of Developmental Biology of Freshwater Fish, Changsha, 410081, China.
School of Animal Science, Yangtze University, Jingzhou, 434020, China; The Key Laboratory of Mariculture (Education Ministry of China), Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, PR China.
Fish Shellfish Immunol. 2019 Jan;84:390-403. doi: 10.1016/j.fsi.2018.10.038. Epub 2018 Oct 15.
In the present study, we identify three type I interferon (IFN) genes (Ad/AsIFNe1-3) and a type II IFN gene (Ad/AsIFNγ) from the Dabry's sturgeon (Acipenser dabryanus) and the Chinese sturgeon (Acipenser sinensis). Sequence analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFNγ contain several conserved characteristics, including signal peptides, interferon alpha, beta, and delta (IFabd) domains, and N-glycosylation sites. Ad/AsIFNe1-3 belongs to the type I IFN group I subgroup, possessing two conserved cysteines residues (C1 and C3), and Ad/AsIFNγ contained a conserved nuclear localization sequence (NLS) motif. Ad/AsIFNe1-3 and Ad/AsIFNγ contain signature motifs indicative of their corresponding IFN group. The Ad/AsIFNe1-3 and Ad/AsIFNγ genes were found to consist of 5 exons/4 introns and 4 exons/3 introns, respectively. These IFNs were separated by four phase 0 introns (type I IFN) and three phase 0 introns (type II IFN). The sequences of IFNe1-3 and IFNγ from the Dabry's sturgeon and the Chinese sturgeon were closely aligned, suggested that these two species are closely related. Phylogenetic analysis revealed that Ad/AsIFNe1-3 and Ad/AsIFNγ clustered together with the corresponding homologous proteins from other fish species. AdIFNe1-3 were found to be high expressed in early embryonic development, suggesting that AdIFNe1-3 might indicate maternal transmission, while AdIFNγ may not mediate embryonic development. Tissue distribution analysis revealed that AdIFNe1-3 and AdIFNγ carry out biological functions in immune and non-immune tissues compartments. AdIFNe1-3 and AdIFNγ can be stimulated by polyinosinic-polycytidylic acid (poly I:C) and lipopolysaccharides (LPS). AdIFNe1-3 have stronger antiviral activity than AdIFNγ, and AdIFNγ has a stronger antibacterial activity than AdIFNe1-3. The differential responses of these genes to poly I:C and LPS suggest differences in the mechanisms of defense against viruses and bacteria.
在本研究中，我们从达氏鲟（Acipenser dabryanus）和中华鲟（Acipenser sinensis）中鉴定出三种 I 型干扰素（IFN）基因（Ad/AsIFNe1-3）和一种 II 型 IFN 基因（Ad/AsIFNγ）。序列分析表明，Ad/AsIFNe1-3 和 Ad/AsIFNγ 具有几个保守特征，包括信号肽、干扰素 α、β 和 δ（IFabd）结构域和 N-糖基化位点。Ad/AsIFNe1-3 属于 I 型 IFN 组 I 亚群，具有两个保守的半胱氨酸残基（C1 和 C3），而 Ad/AsIFNγ 包含一个保守的核定位序列（NLS）基序。Ad/AsIFNe1-3 和 Ad/AsIFNγ 包含与其相应 IFN 组对应的特征基序。Ad/AsIFNe1-3 和 Ad/AsIFNγ 基因分别由 5 个外显子/4 个内含子和 4 个外显子/3 个内含子组成。这些 IFN 由四个相位 0 内含子（I 型 IFN）和三个相位 0 内含子（II 型 IFN）隔开。达氏鲟和中华鲟的 IFNe1-3 和 IFNγ 序列紧密对齐，表明这两个物种密切相关。系统发育分析表明，Ad/AsIFNe1-3 和 Ad/AsIFNγ 与来自其他鱼类物种的相应同源蛋白聚类在一起。AdIFNe1-3 在早期胚胎发育中高表达，表明 AdIFNe1-3 可能指示母体传递，而 AdIFNγ 可能不介导胚胎发育。组织分布分析表明，AdIFNe1-3 和 AdIFNγ 在免疫和非免疫组织中发挥生物学功能。AdIFNe1-3 和 AdIFNγ 可被多聚肌苷酸-多聚胞苷酸（poly I:C）和脂多糖（LPS）刺激。AdIFNe1-3 具有比 AdIFNγ 更强的抗病毒活性，而 AdIFNγ 具有比 AdIFNe1-3 更强的抗细菌活性。这些基因对 poly I:C 和 LPS 的不同反应表明它们在抗病毒和抗细菌防御机制上存在差异。
