Westly H J, Kelley K W
Proc Soc Exp Biol Med. 1987 Jun;185(2):211-8. doi: 10.3181/00379727-185-42537.
Activation of porcine splenocytes with the mitogen, concanavalin A, increases the number of glucocorticoid and beta-adrenergic receptors with no change in the apparent dissociation constant. Incubation of splenocytes with concanavalin A in the presence of hydrocortisone 21-sodium succinate prevented this mitogen-induced increase in glucocorticoid receptors. Isoproterenol also prevented the concanavalin A-induced increase in beta-adrenoceptors at 24 hr and reduced the binding affinity of these receptors at 48 hr. Neither agonist had any significant effect on the receptor number of binding affinity of nonstimulated cells. These data demonstrate that the increase in the number of glucocorticoid and beta-adrenergic receptors that occur on lymphoid cells after activation by a T-cell mitogen can be prevented by appropriate hormone agonists. Down-regulation of receptor number by appropriate agonists appears to be a common regulatory system that is shared by both the neuroendocrine and the immune systems.
用促细胞分裂剂伴刀豆球蛋白A激活猪脾细胞，可增加糖皮质激素受体和β-肾上腺素能受体的数量，而表观解离常数无变化。在21-琥珀酸钠氢化可的松存在的情况下，将脾细胞与伴刀豆球蛋白A一起孵育，可阻止这种促细胞分裂剂诱导的糖皮质激素受体增加。异丙肾上腺素在24小时时也可阻止伴刀豆球蛋白A诱导的β-肾上腺素能受体增加，并在48小时时降低这些受体的结合亲和力。两种激动剂对未刺激细胞的受体数量或结合亲和力均无显著影响。这些数据表明，T细胞促细胞分裂剂激活后淋巴细胞上糖皮质激素受体和β-肾上腺素能受体数量的增加可被适当的激素激动剂阻止。适当的激动剂对受体数量的下调似乎是神经内分泌系统和免疫系统共有的一种常见调节系统。
