Division of Laboratory and Genomic Medicine, Barnes Jewish Hospital, Washington University in St. Louis School of Medicine, 660 South Euclid Avenue, CB 8118, St Louis, MO 63110, USA.
Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Washington University in St. Louis School of Medicine, 660 South Euclid Avenue, CB 8208, St Louis, MO 63110, USA.
Gastroenterol Clin North Am. 2018 Dec;47(4):793-812. doi: 10.1016/j.gtc.2018.07.006. Epub 2018 Sep 29.
Multiplex nucleic acid testing is increasingly used to diagnose childhood gastroenteritis. The advantages of this disruptive technology include rapidity, sensitivity, and ability to detect pathogenic viruses, bacteria, and parasites simultaneously. The drawbacks are its capacity to identify organisms of uncertain clinical significance in North American children, cost, and inability to provide viable bacteria for strain typing by public health authorities. However, this technology will certainly improve our knowledge of the causes of human gut infections. As data emerge, physicians should interpret results cautiously, and, most important, consider the context of the presentation before making clinical decisions based on the readouts.
多重核酸检测越来越多地用于诊断儿童肠胃炎。这项颠覆性技术的优点包括快速、敏感，并且能够同时检测致病病毒、细菌和寄生虫。缺点是它在北美儿童中识别具有不确定临床意义的生物体的能力、成本以及无法为公共卫生部门提供用于菌株分型的活菌。然而，这项技术肯定会增进我们对人类肠道感染原因的了解。随着数据的出现，医生应该谨慎解读结果，最重要的是，在根据检测结果做出临床决策之前，要考虑到临床表现的背景。
