冷等离体子体癌症治疗中的细胞激活现象。
The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment.
机构信息
Department of Mechanical and Aerospace Engineering, The George Washington University, Science & Engineering Hall, 800 22nd Street, NW, Washington, DC, 20052, USA.
State Key Laboratory of Electrical Insulation and Power Equipment, Xi'an Jiaotong University, Xi'an, 710049, China.
出版信息
Sci Rep. 2018 Oct 18;8(1):15418. doi: 10.1038/s41598-018-33914-w.
Cold Atmospheric Plasma (CAP) is an ionized gas with a near room temperature. CAP is a controllable source for reactive species, neutral particles, electromagnetic field and UV radiation. CAP showed the promising application in cancer treatment through the demonstration in vitro and in vivo. In this study, we first demonstrate the existence of an activation state on the CAP-treated cancer cells, which drastically decreases the threshold of cell vulnerability to the cytotoxicity of the CAP-originated reactive species such as HO and NO. The cytotoxicity of CAP treatment is still dependent on the CAP-originated reactive species. The activation state of cancer cells will not cause noticeable cytotoxicity. This activation is an instantaneous process, started even just 2 s after the CAP treatment begins. The noticeable activation on the cancer cells starts 10-20 s during the CAP treatment. In contrast, the de-sensitization of activation takes 5 hours after the CAP treatment. The CAP-based cell activation explains the mechanism by which direct CAP treatment causes a much stronger cytotoxicity over the cancer cells compared with an indirect CAP treatment do, which is a key to understand what the effect of CAP on cancer cells.
冷等离子体（CAP）是一种具有接近室温的离子化气体。CAP 是活性物质、中性粒子、电磁场和紫外辐射的可控源。CAP 在体外和体内的研究中表现出了在癌症治疗方面的应用前景。在这项研究中，我们首先证明了 CAP 处理的癌细胞存在激活状态，这大大降低了细胞对 CAP 衍生的活性物质（如 HO 和 NO）的细胞易感性的阈值。CAP 处理的细胞毒性仍然依赖于 CAP 衍生的活性物质。CAP 处理引起的激活状态不会导致明显的细胞毒性。这种激活是一个瞬间的过程，甚至在 CAP 处理开始后 2 秒就开始了。在 CAP 处理期间，大约 10-20 秒时，癌细胞上会出现明显的激活。相比之下，激活的脱敏作用发生在 CAP 处理后 5 小时。基于 CAP 的细胞激活解释了直接 CAP 处理对癌细胞产生更强细胞毒性的机制，而间接 CAP 处理则不会，这是理解 CAP 对癌细胞影响的关键。
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