Lieberman J, Krauthammer M, Sastre A
Sarcoidosis. 1986 Mar;3(1):60-6.
An animal model with pulmonary, non-caseating, granulomatosis was successfully reproduced in rabbits utilizing intravenous injections of complete-Freund's-adjuvant (CFA) or BCG. Granuloma formation from CFA injections was limited to the lungs, but BCG injections caused systemic granulomatosis (lung, liver and spleen) irrespective of whether the BCG was dissolved in saline or in incomplete-Freund's-adjuvant (IFA). Serum angiotensin-converting-enzyme (ACE) levels began to rise approximately 15 days following the intravenous injection of CFA, but similar rises following the injection of IFA without granuloma formation suggest that the ACE rise was not necessarily reflective of granuloma formation in rabbits. BCG in saline caused no rise in serum ACE, but when suspended in IFA the ACE rise was comparable to that resulting from IFA alone. However, the ACE level of broncho-alveolar-lavage cells obtained from rabbits 21 days post-injection of CFA was 7-fold higher than that of lavaged cells obtained 42 days post injection, correlating with an acute alveolitis occurring early during granuloma formation. These studies confirm the usefulness of intravenous injections of CFA or BCG for inducing a granulomatous state, but show that the assay of serum ACE is not useful for reflecting the granulomatous state in rabbits. In addition, serum ACE levels are 2-5 X higher in rabbits than in humans and tend to fluctuate spontaneously following delivery of the animals to the laboratory.
通过静脉注射完全弗氏佐剂（CFA）或卡介苗（BCG），成功在兔子身上复制出了一种肺部非干酪样肉芽肿病的动物模型。CFA注射所致的肉芽肿形成仅限于肺部，但BCG注射则导致全身性肉芽肿病（肺部、肝脏和脾脏），无论BCG是溶解于生理盐水还是不完全弗氏佐剂（IFA）中。静脉注射CFA后约15天，血清血管紧张素转换酶（ACE）水平开始升高，但注射IFA后无肉芽肿形成时也出现类似升高，这表明ACE升高不一定反映兔子体内的肉芽肿形成。生理盐水配制的BCG未引起血清ACE升高，但悬浮于IFA中时，ACE升高与单独使用IFA时相当。然而，注射CFA后21天从兔子获取的支气管肺泡灌洗细胞的ACE水平比注射后42天获取的灌洗细胞高7倍，这与肉芽肿形成早期出现的急性肺泡炎相关。这些研究证实了静脉注射CFA或BCG诱导肉芽肿状态的有效性，但表明检测血清ACE对反映兔子的肉芽肿状态并无用处。此外，兔子的血清ACE水平比人类高2至5倍，且将动物运至实验室后，其血清ACE水平往往会自发波动。
