Xie T T, Zeng H, Ren X P, Wang N, Chen Z J, Zhang Y, Chen W
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps/College of Life Sciences, Tarim University, Alar, China.
College of Animal Sciences/Key Laboratory of Tarim Animal Husbandy & Science Technology of Xinjiang Production & Construction Corps, Tarim University, Alar, China.
Lett Appl Microbiol. 2019 Jan;68(1):73-80. doi: 10.1111/lam.13087. Epub 2018 Nov 22.
This study sought to identify novel and nontoxic biofilm inhibitors from the Actinomycete library for attenuating biofilm formation by Staphylococcus epidermidis. After investigating the antibiofilm activities of spent media from 185 Actinomycete strains using two S. epidermidis strains (ATCC 35984 and a clinical strain 5-121-2) as target bacteria, three strains of tested Actinomycete (TRM 46200, TRM 41337, and TRM 46814) showed a significant inhibition against S. epidermidis biofilm formation without affecting the growth of planktonic cells. The characteristics of three strains of supernatants suggested that hydrophilic compound possibly extracellular peptides or proteins from these three strains, confer the biofilm reduction in S. epidermidis. An attempt was made to assess their effects on biofilm components and cell surface hydrophobicities in order to disclose acting mechanisms. The crude proteins from spent media of three strains degraded not only exopolysaccharides but also extracellular DNA in S. epidermidis biofilm. The active substances in crude proteins caused S. epidermidis cells to become less hydrophobic. Given these results, the metabolites from Actinomycete strains should keep further attention as potential antibiofilm agents against biofilm formation of S. epidermidis, even biofilm infections of the other bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: Staphylococcus epidermidis infections are frequently associated with biofilms that are difficult to eradicate with conventional antibiotics. The new biofilm inhibitors from Actinomycete will have a great value in the prevention and treatment of dairy cow mastitis and other biofilm-related infections.
本研究旨在从放线菌文库中鉴定新型无毒生物膜抑制剂，以减弱表皮葡萄球菌生物膜的形成。以两株表皮葡萄球菌（ATCC 35984和临床菌株5-121-2）为靶细菌，研究了185株放线菌菌株的发酵液的抗生物膜活性，三株受试放线菌（TRM 46200、TRM 41337和TRM 46814）对表皮葡萄球菌生物膜形成表现出显著抑制作用，且不影响浮游细胞的生长。三株菌的上清液特性表明，这些菌株可能产生的亲水性化合物，即细胞外肽或蛋白质，可减少表皮葡萄球菌生物膜的形成。为揭示其作用机制，尝试评估它们对生物膜成分和细胞表面疏水性的影响。三株菌发酵液中的粗蛋白不仅能降解表皮葡萄球菌生物膜中的胞外多糖，还能降解细胞外DNA。粗蛋白中的活性物质使表皮葡萄球菌细胞的疏水性降低。基于这些结果，放线菌菌株的代谢产物作为潜在的抗生物膜剂，对抑制表皮葡萄球菌生物膜形成乃至其他细菌的生物膜感染应予以进一步关注。研究的意义和影响：表皮葡萄球菌感染常与生物膜有关，传统抗生素难以根除。来自放线菌的新型生物膜抑制剂在预防和治疗奶牛乳腺炎及其他生物膜相关感染方面具有重要价值。
