Mu Yongqi, Zeng Hong, Chen Wei
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, China.
College of Life Sciences, Tarim University, Alar, China.
Front Microbiol. 2021 Mar 4;12:631058. doi: 10.3389/fmicb.2021.631058. eCollection 2021.
is an opportunistic pathogen, and its biofilm formation ability is an important virulent factor. Quercetin, a typical flavonoid ubiquitously used in dietary supplementation, is known for its antioxidant property, but its anti-biofilm activity against remains unknown. In this study, the anti-biofilm activity of quercetin was investigated using ATCC35984, a strong biofilm-positive strain. An attempt was made to disclose the mechanisms of the anti-biofilm activity of quercetin. exhibited a less cell surface hydrophobicity after quercetin treatment. Also, quercetin effectively inhibited cells from adhering to the glass slides. Quercetin downregulated the intercellular adhesion () locus and then polysaccharide intercellular adhesin (PIA) production was reduced. Therefore, cells became less hydrophobic, which supported quercetin's anti-biofilm effect. Our study suggests that quercetin from plants be given further attention as a potential anti-biofilm agent against the biofilm formation of , even biofilm infections of other bacteria.
是一种机会致病菌，其生物膜形成能力是一个重要的致病因素。槲皮素是一种典型的黄酮类化合物，广泛用于膳食补充剂，以其抗氧化特性而闻名，但其对的抗生物膜活性尚不清楚。在本研究中，使用生物膜阳性强菌株ATCC35984研究了槲皮素的抗生物膜活性。试图揭示槲皮素抗生物膜活性的机制。槲皮素处理后表现出较低的细胞表面疏水性。此外，槲皮素有效地抑制细胞粘附到载玻片上。槲皮素下调细胞间粘附（）位点，然后多糖细胞间粘附素（PIA）的产生减少。因此，细胞疏水性降低，这支持了槲皮素的抗生物膜作用。我们的研究表明，植物来源的槲皮素作为一种潜在的抗生物膜剂，可用于对抗的生物膜形成，甚至其他细菌的生物膜感染，应得到进一步关注。
