基于乳铁蛋白标记的量子点的治疗性纳米胶囊，用于联合 COX-2 抑制剂/草药治疗乳腺癌。
Lactoferrin-tagged quantum dots-based theranostic nanocapsules for combined COX-2 inhibitor/herbal therapy of breast cancer.
机构信息
Cancer Nanotechnology Research Laboratory (CNRL), Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
出版信息
Nanomedicine (Lond). 2018 Oct;13(20):2637-2656. doi: 10.2217/nnm-2018-0196. Epub 2018 Oct 19.
AIM
Herein, tumor-targeted quantum dots (QDs)-based theranostic nanocapsules (NCs) coloaded with celecoxib and honokiol were developed. Materials & methodology: The anionic CD44-targeting chondroitin sulfate and cationic low density lipoprotein (LDL)-targeting lactoferrin (LF) were sequentially assembled onto the surface of the positively charged oily core. As an imaging probe, highly fluorescent mercaptopropionic acid-capped cadmium telluride QDs were coupled to LF.
RESULTS
In vitro, fluorescence of QDs was quenched (OFF state) due to combined electron/energy transfer-mediated processes involving LF. After intracellular uptake of NCs, fluorescence was restored (ON state), thus enabled tracing their internalization. The NCs demonstrated enhanced cytotoxicity against breast cancer cells as well as superior in vivo antitumor efficacy.
CONCLUSION
We propose these multifunctional nanotheranostics for imaging and targeted therapy of breast cancer.
目的
在此，开发了载有塞来昔布和和厚朴酚的肿瘤靶向量子点（QD）治疗性纳米胶囊（NC）。
材料与方法
阴离子 CD44 靶向硫酸软骨素和阳离子低密度脂蛋白（LDL）靶向乳铁蛋白（LF）依次组装到带正电荷的油性核心表面。作为成像探针，将高度荧光巯基丙酸封端的碲化镉 QD 与 LF 偶联。
结果
体外，由于涉及 LF 的电子/能量转移介导过程，QD 的荧光被猝灭（OFF 状态）。NC 被细胞内摄取后，荧光恢复（ON 状态），从而能够追踪其内化。NC 对乳腺癌细胞表现出增强的细胞毒性以及优异的体内抗肿瘤功效。
结论
我们提出了这些多功能的纳米治疗药物，用于乳腺癌的成像和靶向治疗。
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