Department of Obstetrics and Gynecology, The First People's Hospital of Wenling, Wenling, Zhejiang, China.
Eur Rev Med Pharmacol Sci. 2018 Oct;22(19):6268-6273. doi: 10.26355/eurrev_201810_16034.
As a gynecological malignant tumor, cervical cancer tends to occur in younger patients. Furin is an important member of precursor protein convertase that may affect malignant tumor metastasis and neovascularization. Pancreatic duodenal homeobox (PDX), as a transcription factor, can inhibit Furin. This study intends to explore the impact of PDX on cervical cancer HeLa cell proliferation and mechanism.
PDX plasmid was transfected to cervical cancer HeLa cells. Cell proliferation, invasion, and migration were tested. HeLa cells were injected to Balb/c nude mice to observe the change of general status, tumor formation rate, tumor weight, and volume.
PDX expression was gradually increased after PDX transfection following time extension. Cell proliferation, invasion, and migration in experimental group were significantly lower than those in normal control (p < 0.05). The nude mice injected with PDX transfected HeLa cells showed markedly better general status, with reducing rate of tumor formation, tumor weight and volume compared with control.
PDX can suppress cervical cancer HeLa cell proliferation, cell invasion and migration, improve general status of tumor-bearing nude mice and reduce tumor weight and volume. Our data highlight the clinical benefits of PDX in inhibiting cervical cancer proliferation, invasion, and metastasis.
宫颈癌作为一种妇科恶性肿瘤，其发病有年轻化趋势。丝氨酸蛋白酶原 2 是前体蛋白转化酶的重要成员之一，可能影响恶性肿瘤的转移和新生血管生成。胰腺十二指肠同源盒（PDX）作为一种转录因子，可以抑制丝氨酸蛋白酶原 2。本研究旨在探讨 PDX 对宫颈癌 HeLa 细胞增殖的影响及其作用机制。
将 PDX 质粒转染至宫颈癌 HeLa 细胞，检测细胞增殖、侵袭和迁移能力。将 HeLa 细胞注入 Balb/c 裸鼠，观察一般状态、肿瘤形成率、肿瘤重量和体积的变化。
PDX 转染后，随时间延长 PDX 表达逐渐增加。实验组细胞增殖、侵袭和迁移能力明显低于正常对照组（p＜0.05）。与对照组相比，转染 PDX 的 HeLa 细胞荷瘤裸鼠一般状态明显改善，肿瘤形成率、肿瘤重量和体积降低。
PDX 可抑制宫颈癌 HeLa 细胞增殖、细胞侵袭和迁移，改善荷瘤裸鼠一般状态，降低肿瘤重量和体积。我们的数据强调了 PDX 在抑制宫颈癌增殖、侵袭和转移方面的临床获益。
