Shi Chong, Zhang Guobin, Han Baosheng, Yang Junhong, Liu Heng, Xi Jinkun
Department of Reproduction and Genetics, Maternal and Child Health Hospital, Tangshan 063000, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Mar;35(3):432-6.
To investigate the effects of the furin inhibitor α1-PDX on the growth, invasion, and tumorigenicity of cervical cancer cells and explore the mechanisms.
The changes in the growth, migration and invasion of α1-PDX-transfected HeLa cells were observed using MTT assay, Boyden migration and invasion assay. The protein levels of furin and MT1-MMP were measured using Western blotting and furin activity was detected by enzyme activity assay in the transfected cells. HeLa cells were seeded subcutaneously in nude mice and the tumor volume changes were recorded.
Compared with the control cells, α1-PDX-treated cells showed a significant growth inhibition by 18.4% at 24 h (P<0.01) with obviously lowered migration ability and cell invasiveness (P<0.01). Treatment with α1-PDX significantly reduced furin enzyme activity and MTI-MMP protein levels in HeLa cells. In nude mice, α1-PDX-treated HeLa cells exhibited a delayed and lowered tumorigenicity with reduced size of the tumors.
α1-PDX can inhibit the growth, metastasis and tumorigenicity of HeLa cells, the mechanism of which may involve a decreased furin activity and MTI-MMP expression.
研究弗林蛋白酶抑制剂α1-PDX对宫颈癌细胞生长、侵袭及致瘤性的影响，并探讨其作用机制。
采用MTT法、Boyden迁移和侵袭实验观察α1-PDX转染的HeLa细胞生长、迁移和侵袭能力的变化。运用蛋白质免疫印迹法检测转染细胞中弗林蛋白酶和MT1-MMP的蛋白水平，通过酶活性测定法检测弗林蛋白酶活性。将HeLa细胞皮下接种于裸鼠，记录肿瘤体积变化。
与对照细胞相比，α1-PDX处理的细胞在24小时时生长受到显著抑制，抑制率为18.4%（P<0.01），迁移能力和细胞侵袭性明显降低（P<0.01）。α1-PDX处理显著降低了HeLa细胞中的弗林蛋白酶活性和MTI-MMP蛋白水平。在裸鼠中，α1-PDX处理的HeLa细胞致瘤性延迟且降低，肿瘤体积减小。
α1-PDX可抑制HeLa细胞的生长、转移及致瘤性，其机制可能与弗林蛋白酶活性降低和MTI-MMP表达减少有关。
