Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, Indiana 47907.
Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, West Lafayette, Indiana 47907.
J Pharm Sci. 2019 Feb;108(2):791-797. doi: 10.1016/j.xphs.2018.10.017. Epub 2018 Oct 16.
In this mini-review, the major types of photolytic labeling reagents are presented together with their reaction mechanisms. The applications of photolytic labeling in protein drug discovery and development are then discussed; these have expanded from studies of protein-protein interactions in vivo to protein-matrix interactions in lyophilized solids. The mini-review concludes with recommendations for further development of the approach, which include the need for new and more chemically diverse photo-reactive reagents and better understanding of the mechanisms of photolytic labeling reactions in various media.
在这篇迷你综述中，介绍了主要类型的光解标记试剂及其反应机制。然后讨论了光解标记在蛋白质药物发现和开发中的应用；这些应用已经从体内的蛋白质-蛋白质相互作用研究扩展到了冻干固体中的蛋白质-基质相互作用。迷你综述最后对该方法的进一步发展提出了建议，包括需要新的和更多化学多样性的光反应试剂，以及更好地理解各种介质中光解标记反应的机制。
