Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Naturais e Biológicas, Universidade Federal do Triangulo Mineiro, Uberaba, Minas Gerais, Brasil.
Microb Biotechnol. 2019 Mar;12(2):265-274. doi: 10.1111/1751-7915.13305. Epub 2018 Oct 19.
Lactococcus lactis is a bacteria with high biotechnological potential, where is frequently used in the amino acid production and production of fermented dairy products, as well as drug delivery systems and mucosal vaccine vector. The knowledge of a functional core proteome is important extremely for both fundamental understanding of cell functions and for synthetic biology applications. In this study, we characterized the L. lacits proteome from proteomic analysis of four biotechnological strains L. lactis: L. lactis subsp. lactis NCDO2118, L. lactis subsp. lactis IL1403, L. lactis subsp. cremoris NZ9000 and L. lactis subsp. cremoris MG1363. Our label-free quantitative proteomic analysis of the whole bacterial lysates from each strains resulted in the characterization of the L. lactis core proteome that was composed by 586 proteins, which might contribute to resistance of this bacterium to different stress conditions as well as involved in the probiotic characteristic of L. lactis. Kegg enrichment analysis shows that ribosome, metabolic pathways, pyruvate metabolism and microbial metabolism in diverse environments were the most enriched. According to our quantitative proteomic analysis, proteins related to translation process were the more abundant in the core proteome, which represent an important step in the synthetic biology. In addition, we identified a subset of conserved proteins that are exclusive of the L. lactis subsp. cremoris or L. lactis subsp. lactis, which some are related to metabolic pathway exclusive. Regarding specific proteome of NCDO2118, we detected 'strain-specific proteins'. Finally, proteogenomics analysis allows the identification of proteins, which were not previously annotated in IL1403 and MG1363. The results obtained in this study allowed to increase our knowledge about the biology of L. lactis, which contributes to the implementation of strategies that make it possible to increase the biotechnological potential of this bacterium.
乳球菌是一种具有高生物技术潜力的细菌，常用于氨基酸生产和发酵乳制品生产，以及药物传递系统和黏膜疫苗载体。功能核心蛋白质组的知识对于细胞功能的基础理解和合成生物学应用都非常重要。在这项研究中，我们通过对四种生物技术菌株乳球菌的蛋白质组学分析来描述乳球菌的蛋白质组：乳球菌亚种乳球菌 NCDO2118、乳球菌亚种乳球菌 IL1403、乳球菌亚种乳脂球菌 NZ9000 和乳球菌亚种乳脂球菌 MG1363。我们对每个菌株的全菌裂解物进行无标记定量蛋白质组学分析，结果描述了由 586 种蛋白质组成的乳球菌核心蛋白质组，这些蛋白质可能有助于该细菌抵抗不同的应激条件，并参与乳球菌的益生菌特性。KEGG 富集分析表明，核糖体、代谢途径、丙酮酸代谢和微生物在各种环境中的代谢是最丰富的。根据我们的定量蛋白质组学分析，与翻译过程相关的蛋白质在核心蛋白质组中更为丰富，这代表了合成生物学的重要步骤。此外，我们鉴定了一组与乳球菌亚种乳脂球菌或乳球菌亚种乳球菌特有代谢途径相关的保守蛋白质。关于 NCDO2118 的特定蛋白质组，我们检测到了“菌株特异性蛋白质”。最后，蛋白质基因组学分析允许鉴定先前在 IL1403 和 MG1363 中未注释的蛋白质。本研究的结果增加了我们对乳球菌生物学的认识，有助于实施增加该细菌生物技术潜力的策略。
