Lehtinen M, Kulomaa P, Lehtinen T
Arch Virol. 1987;94(1-2):71-9. doi: 10.1007/BF01313726.
We studied the expression of the Herpes Simplex virus type-2 (HSV-2) specified major DNA-binding protein (ICSP 11/12) in virus infected RAJI and VERO cells. Immunofluorescence staining by a specific ICSP 11/12 antiserum distinguished between different cell-virus interactions. ICSP 11/12 synthesized in the HSV-2 infected VERO cells showed dense homogenous nuclear staining early in the infection. During virus replication the pattern changed into a less dense, somewhat marginating staining. The corresponding protein synthesized during persistent HSV-2 infection in the RAJI cells showed granular cytoplasmic staining. Late in the infection a whole cell fluorescence was noted. In the absence of virus DNA-synthesis (PFA treated VERO cells) ICSP 11/12 showed dense homogenous or speckled nuclear pattern. The ICSP 11/12 protein extracted from the different virus-cell interactions also showed different elution from double stranded DNA.
我们研究了单纯疱疹病毒2型（HSV-2）特异性主要DNA结合蛋白（ICSP 11/12）在病毒感染的RAJI和VERO细胞中的表达情况。用特异性ICSP 11/12抗血清进行免疫荧光染色可区分不同的细胞-病毒相互作用。在HSV-2感染的VERO细胞中合成的ICSP 11/12在感染早期显示出致密均匀的核染色。在病毒复制过程中，染色模式变为密度较低、有点边缘化的染色。在RAJI细胞中持续HSV-2感染期间合成的相应蛋白显示出颗粒状细胞质染色。在感染后期，观察到全细胞荧光。在没有病毒DNA合成的情况下（经多聚甲醛处理的VERO细胞），ICSP 11/12显示出致密均匀或斑点状的核模式。从不同的病毒-细胞相互作用中提取的ICSP 11/12蛋白从双链DNA上的洗脱情况也有所不同。
