HSV infected RAJI-cells specify HSV specific immediate early and/or early DNA-binding proteins.

Herpes Simplex Virus specified DNA-binding proteins were purified from virus infected VERO and RAJI cells. The expression of the proteins differed depending on the type of the host cell. Polypeptides corresponding to HSV-1 specific ICP 8 and HSV-2 specific ICSP 11/12 were the major products of HSV-infected VERO cells. In RAJI cells polypeptides with a corresponding molecular weight, together with a cellular protein having almost similar mobility on SDS gels could be detected. Using immuno-blotting technique the HSV origin of these 135K molecular weight proteins synthesized in the HSV-1 and HSV-2-infected RAJI cells could be confirmed.