Lehtinen M
Arch Virol. 1986;87(1-2):107-18. doi: 10.1007/BF01310547.
Herpes Simplex Virus specified DNA-binding proteins were purified from virus infected VERO and RAJI cells. The expression of the proteins differed depending on the type of the host cell. Polypeptides corresponding to HSV-1 specific ICP 8 and HSV-2 specific ICSP 11/12 were the major products of HSV-infected VERO cells. In RAJI cells polypeptides with a corresponding molecular weight, together with a cellular protein having almost similar mobility on SDS gels could be detected. Using immuno-blotting technique the HSV origin of these 135K molecular weight proteins synthesized in the HSV-1 and HSV-2-infected RAJI cells could be confirmed.
从感染病毒的VERO细胞和RAJI细胞中纯化出单纯疱疹病毒特异性DNA结合蛋白。这些蛋白的表达因宿主细胞类型而异。对应于单纯疱疹病毒1型特异性ICP 8和单纯疱疹病毒2型特异性ICSP 11/12的多肽是感染单纯疱疹病毒的VERO细胞的主要产物。在RAJI细胞中，可以检测到具有相应分子量的多肽，以及在SDS凝胶上迁移率几乎相似的一种细胞蛋白。使用免疫印迹技术，可以证实这些在感染单纯疱疹病毒1型和2型的RAJI细胞中合成的分子量为135K的蛋白来源于单纯疱疹病毒。
