Department of Urology, Hunan Provincial People's Hospital, the First Affiliated Hospital of Hunan Normal University, Changsha 410005, P.R. China.
Department of Surgery, Hunan Provincial People's Hospital, the First Affiliated Hospital of Hunan Normal University, Changsha 410005, P.R. China.
Int J Biol Macromol. 2019 Feb 1;122:95-103. doi: 10.1016/j.ijbiomac.2018.10.101. Epub 2018 Oct 17.
In this study, a homogeneous polysaccharide (GTP), with a molecular weight of 7.0 × 10 Da, was isolated from Green tea, which was only composed of glucose. The antitumor effects of GTP on prostate cancer (PC) cell line along with the possible mechanism was examined. First, we investigate the potential role of microRNA-93 (miR-93) in PC progression. Our results showed that miR-93 was significantly upregulated in human PC tissues and several PC cell lines, and its overexpression was correlated with poor survival in PC patients. Furthermore, functional analysis showed that miR-93 overexpression promoted the migration, invasion and proliferation of PC-3 cells transfected with miR-93 mimics, while its knockdown displayed an opposite result in DU145 cells following miR-93 inhibitor transfection. Additionally, in vivo tumorigenic studies on nude mice confirmed that miR-93 mimic treatment accelerated the growth of PC-3 xenograft tumors. As expected, GTP (25, 50 and 100 μg/ml) inhibited growth of PC-3 cells via inducing apoptosis, which was achieved by elevation of bax/bcl-2 ratio and caspae-3 protein expression, as well as a decrease of miR-93. Thus, miR-93 may be a potential therapeutic target by GTP for PC therapy.
在这项研究中，从绿茶中分离出一种均相多糖（GTP），其分子量为 7.0×10 Da，仅由葡萄糖组成。研究了 GTP 对前列腺癌（PC）细胞系的抗肿瘤作用及其可能的机制。首先，我们研究了 microRNA-93（miR-93）在 PC 进展中的潜在作用。研究结果表明，miR-93 在人 PC 组织和几种 PC 细胞系中显著上调，其过表达与 PC 患者的不良生存相关。此外，功能分析表明，miR-93 过表达促进了转染 miR-93 模拟物的 PC-3 细胞的迁移、侵袭和增殖，而在 DU145 细胞中转染 miR-93 抑制剂后，其敲低则显示出相反的结果。此外，裸鼠体内致瘤研究证实，miR-93 模拟物处理加速了 PC-3 异种移植肿瘤的生长。正如预期的那样，GTP（25、50 和 100μg/ml）通过诱导细胞凋亡抑制 PC-3 细胞的生长，这是通过升高 bax/bcl-2 比值和 caspase-3 蛋白表达以及降低 miR-93 来实现的。因此，miR-93 可能是 GTP 治疗 PC 的潜在治疗靶点。
