Laboratory of Physiology, Pharmacology and Environmental Health, Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, Fez, Morocco.
New York Medical care For Nephrology, Richmond Hill, New York, USA.
Arch Med Res. 2018 Jul;49(5):306-313. doi: 10.1016/j.arcmed.2018.09.011. Epub 2018 Oct 17.
BACKGROUND/AIM: Various honey samples exhibited protective effect against drug and chemical induced toxicity. The study was designed to determine the antioxidant content and activity of carob honey and to investigate its hepato-renal protective effect in carbon tetrachloride (CCl4) induced kidney and liver injury in rats.
Phenolic, flavone and flavonol in carob honey were quantified. DPPH, ABTS•+, ferric reducting antioxidant power, and total antioxidant activity were used to evaluate the antioxidant activity. Rats were used for the experiment, and received either intraperitoneal injection of CCl4 (1 mL/kg.b.wt); honey (orally, 2 g/kg.b.wt) and CCl4; or honey. Liver and kidney function parameters were assessed. Oxidative parameters including lipid peroxidation (MDA), protein carbonyl formation (PCO), advanced protein oxidation products (AOPP), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and ascorbic acid were measured in the kidney and liver tissues.
CCl4 caused a significant elevation of liver enzymes, lactic acid dehydrogenase, blood glucose, uric acid, blood urea and serum creatinine as compared to the control group. Also, it significantly increased MDA, PCO and AOPP level, and markedly decreased GHS, ascorbic acid, CAT and GPx in the liver and kidney tissues. These changes were significantly ameliorated by carob honey before and after CCl4 administration. Honey alone did not cause significant changes as compared to the control group.
The data showed for the first time that carob honey has high antioxidant content, antioxidant property, and protective effect against CCl4 induced kidney and liver toxicity by maintaining the activity of antioxidant defense system.
背景/目的：各种蜂蜜样品对药物和化学诱导的毒性表现出保护作用。本研究旨在测定角豆蜂蜜的抗氧化含量和活性，并研究其对角叉菜胶（CCl4）诱导的大鼠肾和肝损伤的肝肾功能保护作用。
对角豆蜂蜜中的酚类、黄酮类和黄酮醇类进行定量。使用 DPPH、ABTS•+、铁还原抗氧化能力和总抗氧化活性来评估抗氧化活性。使用大鼠进行实验，腹腔注射 CCl4（1 mL/kg.b.wt）；给予蜂蜜（口服，2 g/kg.b.wt）和 CCl4；或给予蜂蜜。评估肝肾功能参数。测量肾和肝组织中的氧化参数，包括丙二醛（MDA）、蛋白羰基形成（PCO）、高级蛋白氧化产物（AOPP）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）和抗坏血酸。
与对照组相比，CCl4 导致肝酶、乳酸脱氢酶、血糖、尿酸、血尿素和血清肌酐显著升高。此外，它还显著增加了 MDA、PCO 和 AOPP 水平，并显著降低了 GHS、抗坏血酸、CAT 和 GPx 在肝和肾组织中的含量。这些变化在给予角豆蜂蜜前后均得到显著改善。与对照组相比，单独使用蜂蜜并未引起显著变化。
数据首次表明，角豆蜂蜜具有高抗氧化含量、抗氧化特性和保护作用，可通过维持抗氧化防御系统的活性来对抗 CCl4 诱导的肾和肝毒性。
