Khalid Madiha, Manzoor Sobia, Ahmad Hassam, Asif Arun, Bangash Tariq Ali, Latif Amer, Jaleel Shahla
Atta-ur-Rahman School of Applied Bio-Sciences, Department of Healthcare Biotechnology, National University of Sciences and Technology, Islamabad, 44000, Pakistan.
HepatopancreatoBiliary Liver Transplant Unit, Shaikh Zayd Hospital, Lahore, 54000, Punjab, Pakistan.
Mol Biol Rep. 2018 Dec;45(6):2625-2630. doi: 10.1007/s11033-018-4432-0. Epub 2018 Oct 20.
The basic idea behind this study was to discover the association and prevalence of purinoceptors in hepatitis C virus (HCV) and non-HCV hepatocellular carcinoma (HCC). Immunohistochemistry was performed to study the expression of P2X4 and P2X7 receptors on ex-planted liver tissue samples that were collected from HCC patients. Antibodies specific for the P2X4 and P2X7 receptors were used to target the specific receptors and secondary antibody was used with 3,3'-diaminobenzidine (DAB) detection system to visualize the color change in case of any positive expression There was a substantial increase in P2X4 receptor expression in HCV induced HCC as compared to non-HCV HCC. Surprisingly, there was no increase in the P2X7 receptor expression in both HCV HCC and non-HCV HCC. We conclude that P2X4 receptor expression was significant in the presence of HCV HCC. This may confirms the potential role of P2X4 receptor in the presence of virus in liver pathology. However insignificant expression of P2X7 receptor may avert our attention towards understanding the role of this receptor in pro-inflammatory and immune responses.
本研究背后的基本理念是发现嘌呤受体在丙型肝炎病毒（HCV）和非HCV肝细胞癌（HCC）中的关联及患病率。对从HCC患者收集的外植肝组织样本进行免疫组织化学，以研究P2X4和P2X7受体的表达。使用针对P2X4和P2X7受体的特异性抗体靶向特定受体，并使用二抗与3,3'-二氨基联苯胺（DAB）检测系统来观察任何阳性表达情况下的颜色变化。与非HCV HCC相比，HCV诱导的HCC中P2X4受体表达有显著增加。令人惊讶的是，HCV HCC和非HCV HCC中P2X7受体表达均未增加。我们得出结论，在HCV HCC存在时P2X4受体表达显著。这可能证实了P2X4受体在肝脏病理学中病毒存在时的潜在作用。然而，P2X7受体的表达不显著可能使我们忽略了对该受体在促炎和免疫反应中作用的理解。
